Cytochrome P450 [E-Book] : In Vitro Methods and Protocols / edited by Zhengyin Yan, Gary W. Caldwell.
This collection explores detailed experimental protocols necessary for setting up a variety of in vitro cytochrome P450 (CYP) assays that are vital in selecting drug candidates in a drug discovery pipeline. Major factors affecting drug metabolism include CYP expression levels, kinetic parameters for...
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Personal Name(s): | Caldwell, Gary W., editor |
Yan, Zhengyin, editor | |
Edition: |
1st edition 2021. |
Imprint: |
New York, NY :
Humana Press,
2021
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Physical Description: |
XI, 379 pages 94 illustrations, 40 illustrations in color (online resource) |
Note: |
englisch |
ISBN: |
9781071615423 |
DOI: |
10.1007/978-1-0716-1542-3 |
Series Title: |
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Methods in Pharmacology and Toxicology
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Classification: |
- Cytochrome P450: In Vitro Methods and Protocols
- Characteristics of Major Drug Metabolizing Cytochrome P450 Enzymes
- Quantitative Determination of Cytochrome P450 Using LC/MS/MS
- Spectral Quantitation of Total Cytochrome P450 in Microsomes Using a Single-Beam Spectrophotometer
- Cytochrome P450 Enzyme Kinetics: Km and Vmax Determinations Using Metabolite Formation and Substrate Depletion Methods
- Cytochrome P450 Inhibition Assay Using Human Liver Microsomes
- Evaluation of Time-Dependent Cytochrome P450 Inhibition Using the Area Under the Curve Shift Method
- Assessing Cytochrome P450 Time Dependent Inhibition (IC50 Shift Assay) Using Both Diluted and Non-Diluted Methods
- Cytochrome P450 Gene Regulation: Reporter Assays to Assess Pregnane X Receptor (PXR, NR1I2) Activation
- Cytochrome P450 Gene Regulation: Reporter Assays to Assess Aryl Hydrocarbon Receptor (HLHE76, AhR) Activation and Antagonism
- Cytochrome P450 Gene Regulation: Reporter Assays to Assess Direct and Indirect Activation of Constitutive Androstane Receptor (CAR, NR1I3)
- Determination of In Vitro Cytochrome P450 Induction Potential Using Cryopreserved Human Hepatocytes
- Assessing Cytochrome P450 3A (CYP3A) Induction and Suppression Using Primary Human Hepatocyte Spheroids
- Cytochrome P450-Mediated Metabolic Stability Assay in Liver Microsomes
- Assessment of Cytochrome P450 Metabolic Clearance Using Hepatocyte Suspension
- Cytochrome P450 Knock-Out Assay with Non-Selective Inhibitors
- Differentiation of Cytochrome P450-Mediated from Non-CYP-Mediated Metabolism: Aldehyde Oxidase and Xanthine Oxidoreductase
- In Vitro Reaction Phenotyping of Cytochrome P450 Enzymes
- Cytochrome P450-Mediated Drug Bioactivation Assay: An Untargeted High Resolution Accurate Mass LC/MS Assay
- Simultaneously Assessing Concentration Changes in 20 Biochemical Pathways as a Result of Drug Dosing and Cytochrome P450 and Non-Cytochrome P450-Mediated Metabolism: An Untargeted Metabolomics GC/MS Assay
- Simultaneously Assessing Concentration Changes in 17 Biochemical Pathways as a Result of Drug Dosing and Cytochrome P450 and Non-Cytochrome P450-Mediated Metabolism: A Quasi-Untargeted Metabolomics LC/MS Assay
- Assessing Amino Acid Concentration Changes as a Result of Drug Dosing and Cytochrome P450 and Non-Cytochrome P450-Mediated Metabolism.