Enhancers and Promoters [E-Book] : Methods and Protocols / edited by Tilman Borggrefe, Benedetto Daniele Giaimo.
This volume contains cutting-edge techniques to study the function of enhancers and promoters in depth. Chapters are divided into six sections and describe enhancer-promoter transcripts, nucleosome occupancy, DNA accessibility, chromatin interactions, protein-DNA interactions, functional analyses, a...
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Full text |
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Personal Name(s): | Borggrefe, Tilman, editor |
Giaimo, Benedetto Daniele, editor | |
Edition: |
1st edition 2021. |
Imprint: |
New York, NY :
Humana Press,
2021
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Physical Description: |
XI, 376 pages 69 illustrations, 57 illustrations in color (online resource) |
Note: |
englisch |
ISBN: |
9781071615973 |
DOI: |
10.1007/978-1-0716-1597-3 |
Series Title: |
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Methods in Molecular Biology ;
2351 |
Subject (ZB): | |
Subject (LOC): | |
Classification: |
- A New Toolbox to Analyze Enhancer-Promoter Functions
- Global Run-on Sequencing (GRO-Seq)
- Illuminating Enhancer Transcription at Nucleotide Resolution with Native Elongating Transcript Sequencing (NET-Seq)
- Low Quantity single strand CAGE (LQ-ssCAGE) Maps Regulatory Enhancers and Promoters
- Analyses of Promoter, Enhancer and Nucleosome Organisation in Mammalian Cells by MNase-Seq
- Measuring Chromatin Accessibility: ATAC-Seq
- High-resolution ChIP-MNase Mapping of Nucleosome Positions at Selected Genomic Loci And Alleles
- Sequential Chromatin Immunoprecipitation to Identify Heterotypic Nucleosomes
- Low Input Targeted Chromatin Capture (low-T2C)
- Proximity Ligation-assisted ChIP-Seq (PLAC-Seq)
- Analysis of Enhancer-promoter Interactions using CAGE and RADICL-Seq technologies
- Using Open Chromatin Enrichment and Network Hi-C (OCEAN-C) to identify open chromatin interactions
- Assessment of 3D Interactions Between Promoters and Distal Regulatory Elements with Promoter Capture Hi-C (PCHi-C)
- Native Chromatin Proteomics (N-ChroP) to Characterize Histone Post-Translational Modification (PTM) Combinatorics At Distinct Genomic Regions
- Using ChIP-SICAP to Identify Proteins That Co-Localize in Chromatin
- Genome-wide profiling of protein-DNA Interactions with Chromatin Endogenous Cleavage And High-Throughput Sequencing (ChEC-Seq)
- Transcriptional Activation Of Heterochromatin by Recruitment of dCas9 Activators
- Deletion of Regulatory Elements with All-in-One CRISPR-Cas9 Vectors
- Simultaneous Tagmentation-based detection of ChIP/ATAC Signal with Bisulfite Sequencing
- Low Input Whole-Genome Bisulfite Sequencing.