Fusarium wilt [E-Book] : Methods and Protocols / edited by Jeffrey Coleman.
This volume provides a collection of molecular protocols detailing the most common and modern techniques on fusarium wilt. Chapters guide readers through methods on initial isolation, molecular-based identification, genome characterization, generation of mutants, and characterization of interactions...
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Full text |
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Personal Name(s): | Coleman, Jeffrey, editor |
Edition: |
1st edition 2022. |
Imprint: |
New York, NY :
Humana Press,
2022
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Physical Description: |
XI, 208 pages 50 illustrations, 40 illustrations in color (online resource) |
Note: |
englisch |
ISBN: |
9781071617953 |
DOI: |
10.1007/978-1-0716-1795-3 |
Series Title: |
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Methods in Molecular Biology ;
2391 |
Subject (LOC): |
- 1. Pure culture and DNA sequence-based identification of Fusarium from symptomatic plants and diverse substrates
- Isolation of high-molecular weight (HMW) DNA from Fusarium oxysporum for long-read sequencing
- Karyotyping of Fusarium oxysporum by pulsed field gel electrophoresis and the germ tube burst method
- RNA-seq data analysis for differential expression
- Quantification and isolation of spontaneous colony growth variants
- Generation of a T-DNA insertion mutant library of Fusarium oxysporum using Agrobacterium tumefaciens
- Targeted gene disruption via CRISPR/Cas9 ribonucleoprotein complexes in Fusarium oxysporum
- CRISPR/Cas9 RNP-mediated gene fusion to assess protein quantification and subcellular localization in Fusarium oxysporum
- In vivo monitoring of cytosolic pH using the ratiometric pH sensor pHluorin
- Multi-pronged investigation of volatile compound-mediated interactions of Fusarium oxysporum with plants, fungi and bacteria
- Isolation of mitochondria from Fusarium species
- In planta gene expression analysis and colonization of Fusarium oxysporum
- Time-lapse imaging of root pathogenesis and fungal proliferation without physically disrupting roots
- High-throughput screening assays to identify plant natural products with antifungal properties against Fusarium oxysporum
- Screening and assessment of pisatin demethylase activity (PDA).-Detection and genotyping of Fusarium oxysporum f. sp. vasinfectum race 4 (VCG0114), the Fusarium wilt pathogen of cotton.