VEGF Signaling [E-Book] : Methods and Protocols /

VEGF Signaling [E-Book] : Methods and Protocols / edited by Lorna R. Fiedler, Caroline Pellet-Many.

This volume provides an updated collection of protocols for manipulating and studying VEGF signaling pathways in vitro and in vivo and aims to present a range of both firmly established and newly emerging technologies. Covering multiple model species, from mouse to zebrafish to human, the book explo...

More

Saved in:
Full text
Fiedler, Lorna R., editor
Pellet-Many, Caroline, editor
2nd edition 2022.
New York, NY : Humana Press, 2022
XII, 353 pages 62 illustrations, 55 illustrations in color (online resource)
englisch
9781071622179
10.1007/978-1-0716-2217-9
Methods in Molecular Biology ; 2475
Cardiovascular system.
Diseases-Causes and theories of causation.
Lymphatics.
Physiology.



  • Opposing Roles of Vascular Endothelial Growth Factor C in Metastatic Dissemination and Resistance to Radio/Chemotherapy: Discussion of Mechanisms and Therapeutic Strategies
  • Identification of VEGF Isoforms in Mouse, Rat, and Zebrafish Using RT-qPCR
  • Multi-Parameter Fluorescence-Activated Cell Sorting of Human Lymphatic Endothelial Cells
  • Absolute Quantification of Plasma Membrane Receptors via Quantitative Flow Cytometry
  • Measurement of VEGF Content in Exosomes and Subsequent Tumor Tubulogenesis and In Vivo Angiogenesis Functional Assays
  • SH2-Domain Protein Isolation Using Synthetic Phosphorylated Peptides to Study VEGFR2 Signaling
  • Monitoring VEGF-Stimulated Calcium Ion Flux in Endothelial Cells
  • Co-Immunoprecipitation Assays
  • Using Immortalized Endothelial Cells to Study the Roles of Adhesion Molecules in VEGF-Induced Signaling
  • RNAscope for VEGF-A Detection in Human Tumor Bioptic Specimens
  • Global MicroRNA Profiling of Vascular Endothelial Cells
  • Endothelial Cell Tube Formation Assay: An In Vitro Model for Angiogenesis
  • Use of a Thin Layer Assay for Assessing the Angiogenic Potential of Endothelial Cells In Vitro
  • VEGF-A165 -Induced Endothelial Cells Chemotactic Migration and Invasion Assays
  • Measuring Mitochondrial Calcium Fluxes in Cardiomyocyte upon Mechanical Stretch-Induced Hypertrophy
  • Simultaneous Measurement of Endothelial Cell Proliferation and Cell Cycle Stage Using Flow Cytometry
  • Ex Vivo Mouse Aortic Ring Angiogenesis Assay
  • Retinal Microvasculature-on-a-Chip for Modeling VEGF-Induced Permeability
  • Preventing VEGF-Mediated Vascular Permeability by Experimentally Potentiating BBB Characteristics in Endothelial Cells
  • The Embryonic Mouse Hindbrain and Postnatal Retina as In Vivo Models to Study Angiogenesis
  • Evaluating VEGF-Induced Vascular Leakage Using the Miles Assay
  • Modulation of VEGFA Signaling during Heart Regeneration in Zebrafish
  • Three-Dimensional Visualization of Blood and Lymphatic Vessels in the Adult Zebrafish Heart by Chemical Clearing
  • Fluorescence-Activated Cell Sorting and Quantitative Real-Time PCR to Reveal VEGF Expressing Macrophage Populations in the Zebrafish Larvae
  • Assessing Molecular Regulation of Vascular Permeability Using a VEGF-Inducible Zebrafish Model.