Histone Methyltransferases [E-Book] : Methods and Protocols / edited by Raphaël Margueron, Daniel Holoch.
This volume provides methods used to investigate histone methyltransferase function. Chapters guide readers through a comprehensive set of approaches that detail phylogenetic diversity, histone demethylase activities in vitro, generating chromatin substrates, auto-methylation, quantification of meta...
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Full text |
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Personal Name(s): | Holoch, Daniel, editor |
Margueron, Raphaël, editor | |
Edition: |
1st edition 2022. |
Imprint: |
New York, NY :
Humana Press,
2022
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Physical Description: |
XVII, 495 pages 72 illustrations, 53 illustrations in color (online resource) |
Note: |
englisch |
ISBN: |
9781071624814 |
DOI: |
10.1007/978-1-0716-2481-4 |
Series Title: |
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Methods in Molecular Biology ;
2529 |
Subject (LOC): |
- Not all is SET for methylation: Evolution of eukaryotic protein methyltransferases
- Detection and quantification of histone methyltransferase activity in vitro
- In Vitro Histone Demethylase Assays
- Preparation and characterization of chromatin templates for histone methylation assays
- Techniques to study automethylation of histone methyltransferases and its functional impact
- Rodriguez Profiling the regulation of histone methylation and demethylation by metabolites and metals
- Determination of histone methyltransferase structure by crystallography
- Determination of histone methyltransferase structures in complex with the nucleosome by cryogenic electron microscopy
- Development and validation of antibodies targeting site-specific histone methylation
- Genetic, genomic and imaging approaches to dissect the role of Polycomb group epigenetic regulators in mice
- Profiling histone methylation in low numbers of cells
- Automated CUT&RUN using the KingFisher Duo Prime
- Bioinformatics methods for ChIP-seq histone analysis
- Characterization of SET-domain histone lysine methyltransferase substrates using a cofactor S-adenosyl-L-methionine surrogate
- Specificity analysis of protein methyltransferases and discovery of novel substrates using SPOT peptide arrays
- Identifying specific protein interactors of nucleosomes carrying methylated histones using mass spectrometr
- Investigating mitotic inheritance of histone post-translational modifications by triple pSILAC coupled to Nascent Chromatin Capture
- Investigating mitotic inheritance of histone modifications using tethering
- Investigating histone modification dynamics by mechanistic computational modelling
- Screening for small molecule inhibitors of histone methyltransferases.