Drosophila [E-Book] : Methods and Protocols / edited by Christian Dahmann.
This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and...
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Full text |
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Personal Name(s): | Dahmann, Christian, editor |
Edition: |
3rd edition 2022. |
Imprint: |
New York, NY :
Humana Press,
2022
|
Physical Description: |
XI, 424 pages 74 illustrations, 69 illustrations in color (online resource) |
Note: |
englisch |
ISBN: |
9781071625415 |
DOI: |
10.1007/978-1-0716-2541-5 |
Series Title: |
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Methods in Molecular Biology ;
2540 |
Subject (LOC): |
- Using FlyBase, a database of Drosophila genes and genetics
- The Q-System: A versatile repressible binary expression system
- Resources and methods for the analysis of microRNA function in Drosophila
- Analysis of single-cell transcriptome data in Drosophila
- Prime editing for precise genome engineering in Drosophila
- CRISPR/Cas9 mediated precise and efficient genome editing in Drosophila
- Tissue-specific CRISPR-Cas9 screening in Drosophila
- CRISPR-based transcriptional activation in Drosophila
- Tracing and manipulating Drosophila cell lineages based on CRISPR: CaSSA and CLADES
- Studying protein function using nanobodies and other protein binders in Drosophila
- Anchor Away: A system for fast inhibition of proteins in Drosophila
- Tagging Drosophila proteins with genetically-encoded fluorophores
- Optogenetic methods to control tissue mechanics in Drosophila
- Optochemical control of cell contractility in Drosophila embryos
- Stiffness measurement of Drosophila egg chambers by atomic force microscopy
- Cultivation and live imaging of Drosophila imaginal discs
- Sample preparation and imaging of the pupal Drosophila abdominal epidermis
- FlyClear: A tissue clearing technique for high-resolution microscopy of Drosophila
- Preparation of Drosophila tissues and organs for transmission electron microscopy
- Segmentation and quantitative analysis of epithelial tissues
- Genetically-encoded sensors to study metabolism in Drosophila.