Genetically Incorporated Non-Canonical Amino Acids [E-Book] : Methods and Protocols / edited by Yu-Hsuan Tsai, Simon J. Elsässer.
This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging fro...
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Full text |
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Personal Name(s): | Elsässer, Simon J., editor |
Tsai, Yu-Hsuan, editor | |
Edition: |
1st edition 2023. |
Imprint: |
New York, NY :
Humana Press,
2023
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Physical Description: |
XI, 286 pages 1 illustration (online resource) |
Note: |
englisch |
ISBN: |
9781071632512 |
DOI: |
10.1007/978-1-0716-3251-2 |
Series Title: |
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Methods in Molecular Biology ;
2676 |
Subject (LOC): |
- Focused Engineering of Pyrrolysyl-tRNA Synthetase-Based Orthogonal Translation Systems for the Incorporation of Various Non-Canonical Amino Acids
- Engineering Homogeneous Photoactive Antibodies Fragments
- Repurposing Photosensitizer Proteins through Genetic Code Expansion to Facilitate Photo-Biocatalysis
- Genetic Encoding of a Fluorescent Non-Canonical Amino Acid as a FRET Donor for Analysis of Deubiquitinase Activities
- Creating Selenocysteine-Specific Reporters Using Inteins
- Protein Expression with Biosynthesized Non-Canonical Amino Acids
- Reprogramming Initiator and Nonsense Codons to Simultaneously Install Three Distinct Non-Canonical Amino Acids into Proteins in E. coli
- Encoding Non-Canonical Amino Acids into Phage Displayed Proteins
- Genetically Encoded Non-Canonical Amino Acids in Proteins to Investigate Lysine Benzoylation
- Semi-Synthesis of Glutamine-Methylated Proteins Enabled by Genetic Code Expansion
- Genetic Code Expansion in Mammalian Cells
- Generation of Amber Suppression Cell Lines Using CRISPR-Cas9
- Genetic Code Expansion in Mammalian Cells through Quadruplet Codon Decoding
- Genetically Encoded 1,2-Aminothiol for Site-Specific Modification of a Cellular Membrane Protein via TAMM Condensation
- Conformational GPCR BRET Sensors Based on Bioorthogonal Labeling of Non-Canonical Amino Acids
- Selective Inhibition of Kinase Activity in Mammalian Cells by Bioorthogonal Ligand Tethering
- Site-Specific Incorporation of Sulfotyrosine into Proteins in Mammalian Cells
- Small Molecule Phosphine Activation of Protein Function in Zebrafish Embryos with an Expanded Genetic Code
- Non-Canonical Amino Acid Incorporation in Mice.