Cilia [E-Book] : Methods and Protocols / edited by Vito Mennella.
This volume covers the latest advancements in the study of ciliary complexity. Protocols cover genomic, proteomic, imaging, and functional analysis of different ciliated tissues and their wide applicability in cilia biology. Chapters in this book primarily focus on methods to study multiciliated cel...
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Full text |
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Personal Name(s): | Mennella, Vito, editor |
Edition: |
1st edition 2024. |
Imprint: |
New York, NY :
Humana Press,
2024
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Physical Description: |
XII, 278 pages 56 illustrations, 50 illustrations in color (online resource) |
Note: |
englisch |
ISBN: |
9781071635070 |
DOI: |
10.1007/978-1-0716-3507-0 |
Series Title: |
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Methods in Molecular Biology ;
2725 |
Subject (LOC): |
- Combination of CRISPR-Cas9-RNP and Single-Cell RNAseq to Identify Cell State-Specific FOXJ1 Functions in the Human Airway Epithelium
- SARS-CoV-2 Infection of Human Primary Nasal Multiciliated Epithelial Cells Grown on Air-Liquid Interface Cultures
- A Chemically Inducible Organelle Rerouting Assay to Probe Primary Cilium Assembly, Maintenance, and Disassembly in Cultured Cells
- Expansion Microscopy of Ciliary Proteins
- Immunolabel-First-Expand-Later Expansion Microscopy Approach using Stable STED Dyes
- Structural Analysis of Sperm Centrioles Using N-STORM
- A Novel Sandwich Method for Serial Block Face SEM Imaging of Airway Multiciliated Epithelium
- Airway Cells 3D Reconstruction via Manual and Machine-Learning Aided Segmentation of Volume EM Datasets
- Endogenous Tagging of Ciliary Genes in Human RPE1 Cells for Live-Cell Imaging
- Live-Imaging Centriole Amplification in Mouse Brain Multiciliated Cells
- Proximity Mapping of Ciliary Proteins by BioID
- Affinity Purification of Intraflagellar Transport (IFT) Proteins in Mice using Endogenous Streptavidin/FLAG Tags
- Primary Human Nasal Epithelial Cell Culture
- BMI1 Transduction of Human Airway Epithelial Cells for Expansion of Proliferation and Differentiation
- High Speed Video Microscopy of Ependymal Cilia in Brain Organotypic and Cell Culture Models
- Measuring Biophysical Properties of Cilia Motility from Mammalian Tissues via Quantitative Video Analysis Methods
- Mucociliary Transport Device Construction and Application to Study Mucociliary Clearance.