Double-Stranded RNA [E-Book] : Methods and Protocols / edited by Xiaofei Cheng, Guanwei Wu.
This detailed volume examines classical and cutting-edge methods involving double-stranded RNA (dsRNA), specifically regarding isolation, visualization, characterization, production, and application. Many protocols, such as co-immunoprecipitation-based isolation of double-stranded RNA-associated pro...
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Full text |
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Personal Name(s): | Cheng, Xiaofei, editor |
Wu, Guanwei, editor | |
Edition: |
1st edition 2024. |
Imprint: |
New York, NY :
Humana Press,
2024
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Physical Description: |
XI, 142 pages 31 illustrations, 20 illustrations in color (online resource) |
Note: |
englisch |
ISBN: |
9781071637029 |
DOI: |
10.1007/978-1-0716-3702-9 |
Series Title: |
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Methods in Molecular Biology ;
2771 |
Subject (LOC): |
- Isolation of Double-Stranded RNAs by Lithium Chloride Fractionation
- Detection of dsRNA by Acridine Orange Staining
- Isolation of dsRNA from Plants by Cellulose Chromatography
- Rapid Purification of dsRNA Using Micro-Spin Cellulose Column
- Analysis of Virus-Induced Double-Stranded RNA in Living Plant Cells by the dRBFC Assay
- Detection of dsRNA with the Fluorescence In Situ Hybridization (FISH) Assay
- Subcellular Colocalization Assay of Host Factors with Viral Replication Complex in the dsRNA Reporter Nicotiana benthamiana
- Production of Double-Stranded RNA Using the Prokaryotic Promoter-Mediated Bidirectional Transcription
- Inducible Expression of dsRNA in Escherichia coli
- In Vivo Production of dsRNA Using Bacteriophage ϕ6 in Pseudomonas syringae Cit7 Cells
- Bacteria-Based Double-Stranded RNA Production to Develop Cost-Effective RNA Interference Application for Insect Pest Management
- Transiently Induce RNA Silencing in Plants Using a Tobacco Necrosis Virus A (TNV-A)-Based dsRNA Production System
- Co-Immunoprecipitation-Based Isolation of Double-Stranded RNA-Associated Protein Complexes in Nicotiana benthamiana
- Analysis of Plant Virus-Induced Immunity by Using Viral-Derived Double-Stranded RNA in Arabidopsis thaliana
- Identification of Mycoviruses by dsRNA Extraction
- Production of Double-Stranded RNA In Planta by a Potato Mop-Top Virus (PMTV)-Based Vector for Inducing Gene Silencing
- Application of dsRNA for Fungi Disease Management Sclerotinia sclerotiorum and Botrytis cinerea
- Application of dsRNA in the Pine Wood Nematode, Bursaphelenchus xylophilus.