Circular RNAs [E-Book] / edited by Christoph Dieterich, Marie-Laure Baudet.
This second edition details new and updated methods on circular RNA. Chapter guide readers through circular RNA purification, in silico characterization, circRNA detection, sequence validation, quantification , techniques related to gain- and loss-of-function approaches, circular RNA synthesis, spli...
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Full text |
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Personal Name(s): | Baudet, Marie-Laure, editor |
Dieterich, Christoph, editor | |
Edition: |
2nd edition 2024. |
Imprint: |
New York, NY :
Humana Press,
2024
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Physical Description: |
XI, 326 pages 58 illustrations, 53 illustrations in color (online resource) |
Note: |
englisch |
ISBN: |
9781071636787 |
DOI: |
10.1007/978-1-0716-3678-7 |
Series Title: |
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Methods in Molecular Biology ;
2765 |
Subject (LOC): |
- Purification of circular RNAs using poly(A) tailing followed by RNase R digestion
- State of the art circular RNA analytics using the circtools software suite
- Exploring circular RNA profile and expression in extracellular vesicles
- Sanger sequencing to determine the full-length sequence of circular RNAs
- Droplet digital PCR for the detection and quantification of bona fide circRNAs
- Targeted sequencing of circular RNAs for Illumina-based counting and Nanopore structure determination
- Nanopore mediated sequencing of circular RNA
- In vivo tissue-specific knockdown of circRNAs using shRNAs in Drosophila Melanogaster
- Functional circular RNA screening via RfxCas13d/BSJ-gRNA system
- Base-editor-mediated circRNA knockout by targeting predominantly back-splice sites
- Directed Circularization of a Short RNA
- Engineering Synthetic circRNAs for Efficient CNS Expression
- Functional investigation of in vitro transcribed circular RNA and formulation into Lipid nanoparticles via microfluidic mixing
- Characterising post-transcriptional modifications of circRNAs to investigate biogenesis and translation
- Native circular RNA pulldown method to simultaneously profile RNA and protein interactions
- Screening and characterization of functional circRNAs in neuronal cultures. .