Mammalian Synthetic Systems [E-Book] / edited by Francesca Ceroni, Karen Polizzi.
This volume details cutting-edge methods that enables engineering of novel functions in mammalian cells. Chapters guide readers through cell-to-cell communication, cell fate control, protein and RNA-based biosensors together with tools for more reliable and faster mammalian genome editing. Written i...
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Full text |
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Personal Name(s): | Ceroni, Francesca, editor |
Polizzi, Karen, editor | |
Edition: |
1st edition 2024. |
Imprint: |
New York, NY :
Humana Press,
2024
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Physical Description: |
XI, 316 pages 88 illustrations (online resource) |
Note: |
englisch |
ISBN: |
9781071637180 |
DOI: |
10.1007/978-1-0716-3718-0 |
Series Title: |
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Methods in Molecular Biology ;
2774 |
Subject (LOC): |
- A directed evolution protocol for engineering minimal transcription factors, based on CIS display
- Set-up and applications of Modular Protein Expression Toolboxes (MoPET) for mammalian systems
- Coiled-coiled interaction toolbox for engineering mammalian cells
- A mammalian-based synthetic biology toolbox to engineer membrane-membrane interfaces
- Engineered protease-responsive RNA Binding Proteins (RBPs) to expand the toolbox of synthetic circuits in mammalian cells
- Mechanistic model-driven biodesign in mammalian synthetic biology
- Realizing Antithetic Integral Feedback Control in Mammalian Cells
- A computational modeling approach for the design of genetic control systems that respond to transcriptional activity
- Flux Balance Analysis of Mammalian Cell Systems
- Multiplex functional characterization of protein variant libraries in mammalian cells with single-copy genomic integration and high throughput DNA sequencing
- Flow Cytometry Quantification of Transient Transfections in Mammalian CellsRNA switches using Cas proteins
- Multiplexed transactivation of mammalian cells using dFnCas12a-VPR
- Anti-CRISPR Proteins and their Application to Control CRISPR-effectors in Mammalian Systems
- Post-translational Remote Control Mediated by Physical Inducers for Rapid Protein Release in Engineered Mammalian Cells
- Detection of microRNAs Using Synthetic Toehold Switch in Mammalian Cells
- Detection of microRNAs Using Synthetic Toehold Switch in Mammalian Cells
- High-throughput spectroscopic analysis of mRNA capping level
- In vitro generation of megakaryocytes from engineered mouse embryonic stem cells
- Preparation of chromobodies for the detection of cell surface epitopes.