Animal Cell Culture: Principles and Practice [E-Book] / by Shalini Mani, Manisha Singh, Anil Kumar.
This introductory guide provides novice researchers and lab students with a thorough step-by-step approach to standard animal cell culture techniques. Coverage includes lab safety and best practices, sterility management, preparation, ethical considerations, and troubleshooting for common pain point...
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Full text |
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| Personal Name(s): | Mani, Shalini, author |
| Kumar, Anil, author / Singh, Manisha, author | |
| Edition: |
1st edition 2023. |
| Imprint: |
Cham :
Springer,
2023
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| Physical Description: |
XVI, 327 pages 91 illustrations, 85 illustrations in color (online resource) |
| Note: |
englisch |
| ISBN: |
9783031194856 |
| DOI: |
10.1007/978-3-031-19485-6 |
| Series Title: |
Techniques in Life Science and Biomedicine for the Non-Expert
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| Subject (LOC): |
- 1. Overview to animal cell culture
- 2. Cell culture laboratory
- 3. Good lab practices and bio-safety measures
- 4. Managing sterility in the lab
- 5. Media and buffer preparation for cell culture
- 6. Properties of cultured cells and selection of culture media
- 7. Selection and maintenance of cultured cells
- 8. Inoculation and passaging of adherent cells
- 9. Counting of cells
- 10. Cryopreservation of cell lines
- 11. Resuscitation of frozen cell line
- 12. Isolation and culturing of cells from different tissues
- 13. Differentiation, transformation and immortalization of cells
- 14. Co-culture techniques
- 15. 3d cell culture techniques
- 16. Stem cell culture techniques
- 17. Identification and removal of biological contamination in the media and cell suspensions
- 18. Analysis of cell growth kinetics in suspension and adherent types cell line
- 19. Applications of animal cell culture based assays
- 20. Ethical issues in animal cell culture
- 21. Common troubleshooting methods in cell culture techniques.