This title appears in the Scientific Report :
2023
Please use the identifier:
http://dx.doi.org/10.1016/j.bios.2022.114219 in citations.
Please use the identifier: http://hdl.handle.net/2128/34137 in citations.
Delineating charge and capacitance transduction in system-integrated graphene-based BioFETs used as aptasensors for malaria detection
Delineating charge and capacitance transduction in system-integrated graphene-based BioFETs used as aptasensors for malaria detection
Despite significant eradication efforts, malaria remains a persistent infectious disease with high mortality due to the lack of efficient point-of-care (PoC) screening solutions required to manage low-density asymptomatic parasitemia. In response, we demonstrate a quantitative electrical biosensor b...
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Personal Name(s): | Figueroa Miranda, Gabriela |
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Liang, Yuanying / Suranglikar, Mohit / Stadler, Matthias / Samane, Nagesh / Tintelott, Marcel / Lo, Young / Tanner, Julian A. / Vu, Duy Tam / Knoch, Joachim / Ingebrandt, Sven / Offenhäusser, Andreas / Pachauri, Vivek / Mayer, Dirk (Corresponding author) | |
Contributing Institute: |
Bioelektronik; IBI-3 |
Published in: | Biosensors and bioelectronics, 208 (2022) S. 114219 |
Imprint: |
Amsterdam [u.a.]
Elsevier Science
2022
|
DOI: |
10.1016/j.bios.2022.114219 |
Document Type: |
Journal Article |
Research Program: |
Molecular Information Processing in Cellular Systems |
Link: |
Published on 2022-03-26. Available in OpenAccess from 2024-03-26. |
Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/34137 in citations.
Despite significant eradication efforts, malaria remains a persistent infectious disease with high mortality due to the lack of efficient point-of-care (PoC) screening solutions required to manage low-density asymptomatic parasitemia. In response, we demonstrate a quantitative electrical biosensor based on system-integrated two-dimensional field-effect transistors (2DBioFETs) of reduced graphene oxide (rGO) as transducer for high sensitivity screening of the main malaria biomarker, Plasmodium falciparum lactate dehydrogenase (PfLDH). The 2DBioFETs were biofunctionalized with pyrene-modified 2008s aptamers as specific PfLDH receptors. While we systematically optimize biosensor interface for optimal performance, aptamer-protein transduction at 2DBioFETs is elucidated based on delineation of charge and capacitance in an updated analytical model for two-dimensional rGO/biofunctional layer/electrolyte (2DiBLE) interfaces. Our 2DBioFET-aptasensors display a limit-of-detection down to 0.78 fM (0.11 pg/mL), dynamic ranges over 9 orders of magnitude (subfemto to submicromolar), high sensitivity, and selectivity in human serum validating their diagnostic potential as rapid PoC tests for malarial management. |