This title appears in the Scientific Report :
2023
Please use the identifier:
http://hdl.handle.net/2128/34215 in citations.
Please use the identifier: http://dx.doi.org/10.3390/genes14020454 in citations.
Temporal and Spatial Gene Expression Profile of Stroke Recovery Genes in Mice
Temporal and Spatial Gene Expression Profile of Stroke Recovery Genes in Mice
Stroke patients show some degree of spontaneous functional recovery, but this is not sufficient to prevent long-term disability. One promising approach is to characterize the dynamics of stroke recovery genes in the lesion and distant areas. We induced sensorimotor cortex lesions in adult C57BL/6J m...
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Personal Name(s): | Götz, Jan |
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Wieters, Frederique / Fritz, Veronika J. / Käsgen, Olivia / Kalantari, Aref / Fink, Gereon Rudolf / Aswendt, Markus (Corresponding author) | |
Contributing Institute: |
Kognitive Neurowissenschaften; INM-3 |
Published in: | Genes, 14 (2023) 2, S. 454 - |
Imprint: |
Basel
MDPI
2023
|
DOI: |
10.3390/genes14020454 |
Document Type: |
Journal Article |
Research Program: |
SFB 1451: Schlüsselmechanismen normaler und krankheitsbedingt gestörter motorischer Kontrolle Multilevel Brain Organization and Variability |
Link: |
OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://dx.doi.org/10.3390/genes14020454 in citations.
Stroke patients show some degree of spontaneous functional recovery, but this is not sufficient to prevent long-term disability. One promising approach is to characterize the dynamics of stroke recovery genes in the lesion and distant areas. We induced sensorimotor cortex lesions in adult C57BL/6J mice using photothrombosis and performed qPCR on selected brain areas at 14, 28, and 56 days post-stroke (P14-56). Based on the grid walk and rotating beam test, the mice were classified into two groups. The expression of cAMP pathway genes Adora2a, Pde10a, and Drd2, was higher in poor- compared to well-recovered mice in contralesional primary motor cortex (cl-MOp) at P14&56 and cl-thalamus (cl-TH), but lower in cl-striatum (cl-Str) at P14 and cl-primary somatosensory cortex (cl-SSp) at P28. Plasticity and axonal sprouting genes, Lingo1 and BDNF, were decreased in cl-MOp at P14 and cl-Str at P28 and increased in cl-SSp at P28 and cl-Str at P14, respectively. In the cl-TH, Lingo1 was increased, and BDNF decreased at P14. Atrx, also involved in axonal sprouting, was only increased in poor-recovered mice in cl-MOp at P28. The results underline the gene expression dynamics and spatial variability and challenge existing theories of restricted neural plasticity.Keywords: behavior; cAMP pathway; grid walk; qPCR; recovery rate; rotating beam test. |