This title appears in the Scientific Report :
2010
Please use the identifier:
http://dx.doi.org/10.1016/j.bios.2010.05.031 in citations.
Extracellular recording of glycine receptor chloride channel activity as a prototype for biohybrid sensors
Extracellular recording of glycine receptor chloride channel activity as a prototype for biohybrid sensors
There is a continuously growing scientific and technological interest to develop and improve the application of artificial sensors. Biological components which are capable to transduce neutral signals into specific, robust and reproducible indicators frame an attractive alternative to construct bioh...
Saved in:
Personal Name(s): | Sommerhage, F. |
---|---|
Baumann, A. / Wrobel, G. / Ingebrandt, S. / Offenhäusser, A. | |
Contributing Institute: |
Bioelektronik; PGI-8 JARA-FIT; JARA-FIT Zelluläre Biophysik; ISB-1 Bioelektronik; ICS-8 |
Published in: | Biosensors and bioelectronics, 26 (2010) S. 155 - 161 |
Imprint: |
Amsterdam [u.a.]
Elsevier Science
2010
|
Physical Description: |
155 - 161 |
PubMed ID: |
20619629 |
DOI: |
10.1016/j.bios.2010.05.031 |
Document Type: |
Journal Article |
Research Program: |
BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung Grundlagen für zukünftige Informationstechnologien |
Series Title: |
Biosensors & Bioelectronics
26 |
Subject (ZB): | |
Publikationsportal JuSER |
There is a continuously growing scientific and technological interest to develop and improve the application of artificial sensors. Biological components which are capable to transduce neutral signals into specific, robust and reproducible indicators frame an attractive alternative to construct biohybrid sensors. Since naturally "occurring" biosensors are only sparsely compatible with artificial devices, genetic engineering of eukaryotic cells provides an attractive approach, where cells can be tailored such to detect target compounds with exquisite specificity and sensitivity. We have developed the prototype for a single-cell-based anion-selective biohybrid sensor. HEK293 cells were stably transfected with a gene encoding glycine receptor alpha(1) subunits. These cells were employed as transducers for glycine-evoked chloride currents in a concentration-dependent way. Cultured on substrate-integrated micro-devices, anionic membrane currents of cells were monitored extracellularly with field-effect transistors (FETs) and gold microelectrode arrays (MEAs). The results supported predictions of state-of-the-art models for cell-sensor coupling mechanisms and confirmed that extracellularly recorded anion currents cause similar signals, regardless whether obtained with field-effect transistors or microelectrodes. The whole-cell sensor successfully tracked glycine concentrations differing by three orders of magnitude. To our knowledge this contribution for the first time marks the functional characterization of an anion-selective biohybrid sensor. |