This title appears in the Scientific Report :
2023
Please use the identifier:
http://dx.doi.org/10.1016/j.neulet.2023.137456 in citations.
Please use the identifier: http://dx.doi.org/10.34734/FZJ-2023-04873 in citations.
2,4-Dinitrophenol does not exert neuro-regenerative potential in experimental autoimmune neuritis
2,4-Dinitrophenol does not exert neuro-regenerative potential in experimental autoimmune neuritis
Objective: We evaluated the potential neuro-regenerative effects of the mitochondrial uncoupler 2,4-Dinitrophenol in experimental autoimmune neuritis, an animal model for an acute autoimmune neuropathy.Methods: Experimental autoimmune neuritis was induced in Lewis rats. Different concentrations of 2...
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Personal Name(s): | Kohle, Felix (Corresponding author) |
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Ackfeld, Robin / Klein, Ines / Svačina, Martin K. R. / Schneider, Christian / van Beers, Tim / Grandoch, Andrea / Fink, Gereon R. / Lehmann, Helmar C. / Barham, Mohammed | |
Contributing Institute: |
Kognitive Neurowissenschaften; INM-3 |
Published in: | Neuroscience letters, 814 (2023) S. 137456 - |
Imprint: |
Amsterdam [u.a.]
Elsevier Science
2023
|
DOI: |
10.1016/j.neulet.2023.137456 |
DOI: |
10.34734/FZJ-2023-04873 |
Document Type: |
Journal Article |
Research Program: |
Multilevel Brain Organization and Variability |
Link: |
Get full text OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://dx.doi.org/10.34734/FZJ-2023-04873 in citations.
Objective: We evaluated the potential neuro-regenerative effects of the mitochondrial uncoupler 2,4-Dinitrophenol in experimental autoimmune neuritis, an animal model for an acute autoimmune neuropathy.Methods: Experimental autoimmune neuritis was induced in Lewis rats. Different concentrations of 2,4-Dinitrophenol (1 mg/kg, 0.1 mg/kg and 0.01 mg/kg) were applied during the recovery phase of the neuritis (at days 18, 22 and 26) and compared to the vehicle. Any effects were assessed through functional, electrophysiological, and morphological analysis via electron microscopy of all groups at day 30. Additional immune-histochemical analysis of inflammation markers and remyelination of the sciatic nerves were performed for the dosage of 1 mg/kg and control.Results: No enhancement of functional or electrophysiological recovery was observed in all 2,4-Dinitrophenol-treated groups. Cellular inflammation markers of T cells (CD3+) were comparable to control, and an increase of macrophages (IbA1+) invasion in the sciatic nerves was observed. Treatment with 2,4-Dinitrophenol reduced axonal swelling in myelinated and unmyelinated fibers with an increased production of brain-derived neurotrophic factor.Conclusion: Our findings do not support the hypothesis that repurposing of the mitochondrial uncoupler 2,4-Dinitrophenol exerts functionally relevant neuro-regenerative effects in autoimmune neuritis.Keywords: Autoimmune neuropathy; Brain-derived neurotrophic factor; Drug repurposing; Guillain-Barré syndrome; Mitochondrial uncoupler; Neuroregeneration. |