This title appears in the Scientific Report :
2023
Understanding the phenotype and molecular response of Brachypodium under nutrient limitation (and microbe assisted improvements of plant performance)
Understanding the phenotype and molecular response of Brachypodium under nutrient limitation (and microbe assisted improvements of plant performance)
Brachypodium is phylogenetically closely related to many crop plants. Because of this and the ease of handling coming from its shorter life-cycle and smaller size, Brachypodium offers the possibility to efficiently address relevant problems in today’s agriculture. However, to fully harness this pote...
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Personal Name(s): | Sanow, Stefan |
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Amini / kuang, weiqi / Mau, Lisa / Kant, Josefine / Huesgen, Pitter / Hanikenne / Roessner / Watt, Michelle / Arsova, Borjana (Corresponding author) | |
Contributing Institute: |
Pflanzenwissenschaften; IBG-2 |
Imprint: |
2023
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Conference: | International Brachypodium Conference, Hammamet (Tunisia), 2023-07-11 - 2023-07-13 |
Document Type: |
Conference Presentation |
Research Program: |
Biological and environmental resources for sustainable use |
Publikationsportal JuSER |
Brachypodium is phylogenetically closely related to many crop plants. Because of this and the ease of handling coming from its shorter life-cycle and smaller size, Brachypodium offers the possibility to efficiently address relevant problems in today’s agriculture. However, to fully harness this potential we need detailed understanding of Brachypodium’s metabolism and physiology. To this aim we need to superimpose responses through space (plant tissue and organ) and time (developmental stage or from moment of abiotic influence) and link them with the plant metabolic and physiological pathways. [1] This can be done by linking non-invasive phenotyping and molecular or “omics” approaches.We present studies on nutrient sensing and signaling focusing on the micronutrient Zinc (Zn) and the macronutrient Nitrogen (N). For Zn, we discovered the existence of a root to shoot signal in Bd21-3 plants subjected to Zn deficiency and re-supply. Namely, wherein Zn starved plants Bd will overexpress Zn transporters in both roots and shoots, several minutes post Zn re-supply in the medium, the transcriptional levels in the shoot are downregulated by the plant. Using elemental analysis and Zn-isotope flux measurements, in combination with transcriptomics and non-invasive phenotyping we explore the pathways how Brachypodium, a monocot plant, responds to sudden Zn availability and link this to the response of the dicot model plant Arabidopsis [2,3,4].N is the second most abundant nutrient necessary for plant growth and the major nutrient taken up from soils. N deficiency has multiple consequences on agricultural yield, while its mitigation via fertilizers exerts a dangerous ecological price and economical burden. We try to understand how plants adapt to the presence of beneficial microbes with potential N-fixing ability, and how this interaction depends on the abiotic enviroment where it takes place. We found that Herbaspirillum seropedicae (Hs) will promote plant growth under low and sufficient N availability in gnotobiotic EcoFAB chambers, but it appears that different promotional pathways take place in the two conditions. The plant shows different expression of transcripts linked to N-metabolism through time, and a changed preference to the ammonium and nitrate ions when inoculated with Hs [5]. Pseudomonas koreensis on the other hand, promotes plant growth at low N availability, while we see a neutral effect at N sufficiency. Proteomics reveals changes in Nitrogen transporters and other enzymes of N and C metabolism, while elemental analysis shows increase on N content in the inoculated plants [6]. Finally, aiming to close the nutrient loop we asked the question whether algae (which could originate e.g. from various waste water sources) can be used as fertilizers to promote plant growth [7]. The inherent problem is that alae produce a nutrient supply of unusual ratios. We will present initial results that point to sufficient P supply, but the situation with N is not clear. 1. Arsova et al., New Phytol (2020) 225: 1111–11192. Amini et al., (2021) Plant Cell Environ 44(10): 3376-3397.3. Amini, et al., (2022). Plant Cell Environ 45(5): 1339-1361.4. Arsova et al., (2019) bioRxiv: 600569.5. Kuang et al., (2022), JExBot, 73(15): 5306-5321.6. Sanow et al., (2023) MPMI doi:10.1094/MPMI-10-22-0223-CR7. Mau et al., (2022) Agronomy 12(2). |