This title appears in the Scientific Report :
2011
Please use the identifier:
http://dx.doi.org/10.1016/j.jbiotec.2010.07.015 in citations.
The pstSCAB operon for phosphate uptake is regulated by the global regulator GlxR in Corynebacterium glutamicum
The pstSCAB operon for phosphate uptake is regulated by the global regulator GlxR in Corynebacterium glutamicum
The pstSCAB operon of Corynebacterium glutamicum, which encodes a high affinity transport system for uptake of the phosphorus source inorganic phosphate, is induced upon phosphate starvation involving activation by the two-component regulatory system PhoS-PhoR. Partial phosphate starvation induction...
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Personal Name(s): | Panhorst, M. |
---|---|
Sorger-Herrmann, U. / Wendisch, V. F. | |
Contributing Institute: |
Biotechnologie 1; IBT-1 |
Published in: | Journal of biotechnology, 154 (2011) |
Imprint: |
Amsterdam [u.a.]
Elsevier Science
2011
|
PubMed ID: |
20638427 |
DOI: |
10.1016/j.jbiotec.2010.07.015 |
Document Type: |
Journal Article |
Research Program: |
Biotechnologie |
Series Title: |
Journal of Biotechnology
154 |
Subject (ZB): | |
Publikationsportal JuSER |
The pstSCAB operon of Corynebacterium glutamicum, which encodes a high affinity transport system for uptake of the phosphorus source inorganic phosphate, is induced upon phosphate starvation involving activation by the two-component regulatory system PhoS-PhoR. Partial phosphate starvation induction of the pstSCAB operon in a ΔphoRS mutant indicated the involvement of (an) additional transcriptional regulator(s). Here, GlxR, a global cAMP-dependent transcriptional regulator, was shown to bind to the pstS promoter -133 bp to -117 bp upstream of the transcriptional start site as shown by gel shift and mutation experiments. Transcriptional fusion analysis revealed that GlxR activates the pstSCAB operon under phosphate limiting conditions in a carbon source dependent manner. Commensurate with these findings, overexpression of glxR was shown to stimulate growth under phosphate limiting conditions with glucose, but not with acetate, as carbon source. Thus, in C. glutamicum pstSCAB expression is regulated in response to the availability of phosphorus and carbon sources. |