This title appears in the Scientific Report :
2010
Please use the identifier:
http://hdl.handle.net/2128/15768 in citations.
Please use the identifier: http://dx.doi.org/10.1039/c003091k in citations.
Light induced stimulation and delay of cardiac activity
Light induced stimulation and delay of cardiac activity
This article shows the combination of light activatable ion channels and microelectrode array (MEA) technology for bidirectionally interfacing cells. HL-1 cultures, a mouse derived cardiomyocyte-like cell line, transfected with channelrhodopsin were stimulated with a microscope coupled 473 nm laser...
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Personal Name(s): | Hofmann, B. |
---|---|
Maybeck, V. / Eick, S. / Meffert, S. / Ingebrandt, S. / Wood, P. / Bamberg, E. / Offenhäusser, A. | |
Contributing Institute: |
Institut für Bio- und Nanosysteme - Bioelektronik; IBN-2 JARA-FIT; JARA-FIT |
Published in: | Lab on a chip, 10 (2010) S. 2588 - 2596 |
Imprint: |
Cambridge
RSC
2010
|
Physical Description: |
2588 - 2596 |
DOI: |
10.1039/c003091k |
PubMed ID: |
20689860 |
Document Type: |
Journal Article |
Research Program: |
BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung Grundlagen für zukünftige Informationstechnologien |
Series Title: |
Lab on a Chip
10 |
Subject (ZB): | |
Link: |
Get full text OpenAccess OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://dx.doi.org/10.1039/c003091k in citations.
This article shows the combination of light activatable ion channels and microelectrode array (MEA) technology for bidirectionally interfacing cells. HL-1 cultures, a mouse derived cardiomyocyte-like cell line, transfected with channelrhodopsin were stimulated with a microscope coupled 473 nm laser and recorded with custom built 64 electrode MEAs. Channelrhodopsin induced depolarization of the cell can evoke action potentials (APs) in single cells. Spreading of the AP over the cell layer can then be measured with good spatiotemporal resolution using MEA recordings. The possibility for light induced pacemaker switching in cultures was shown. Furthermore, the suppression of APs can also be achieved with the laser. Possible applications include cell analysis, e.g. pacemaker interference or induced pacemaker switching, and medical applications such as a combined cardiac pacemaker and defibrillator triggered by light. Since current prosthesis research focuses on bidirectionality, this system may be applied to any electrogenic cell, including neurons or muscles, to advance this field. |