This title appears in the Scientific Report :
2010
Please use the identifier:
http://dx.doi.org/10.1039/C0CP01048K in citations.
Specific cellular water dynamics observed in vivo by neutron scattering and NMR
Specific cellular water dynamics observed in vivo by neutron scattering and NMR
Neutron scattering, by using deuterium labelling, revealed how intracellular water dynamics, measured in vivo in E. coli, human red blood cells and the extreme halophile, Haloarcula marismortui, depends on the cell type and nature of the cytoplasm. The method uniquely permits the determination of mo...
Saved in:
Personal Name(s): | Jasnin, M. |
---|---|
Stadler, A. / Tehei, M / Zaccai, G. | |
Contributing Institute: |
Molekulare Biophysik; ISB-2 |
Published in: | Physical Chemistry Chemical Physics, 12 (2010) S. 10154 - 10160 |
Imprint: |
Cambridge
RSC Publ.
2010
|
Physical Description: |
10154 - 10160 |
PubMed ID: |
20714607 |
DOI: |
10.1039/C0CP01048K |
Document Type: |
Journal Article |
Research Program: |
BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung |
Series Title: |
Physical Chemistry Chemical Physics
12 |
Subject (ZB): | |
Publikationsportal JuSER |
Neutron scattering, by using deuterium labelling, revealed how intracellular water dynamics, measured in vivo in E. coli, human red blood cells and the extreme halophile, Haloarcula marismortui, depends on the cell type and nature of the cytoplasm. The method uniquely permits the determination of motions on the molecular length (approximately ångstrøm) and time (pico- to nanosecond) scales. In the bacterial and human cells, intracellular water beyond the hydration shells of cytoplasmic macromolecules and membrane faces flows as freely as liquid water. It is not "tamed" by confinement. In contrast, in the extreme halophile archaeon, in addition to free and hydration water an intracellular water component was observed with significantly slowed down translational diffusion. The results are discussed and compared to observations in E. coli and Haloarcula marismortui by deuteron spin relaxation in NMR--a method that is sensitive to water rotational dynamics on a wide range of time scales. |