This title appears in the Scientific Report :
2010
Please use the identifier:
http://hdl.handle.net/2128/15191 in citations.
Metabolische 13C-Stoffflussanalyse - vom isotopisch stationären zum instationären Fall
Metabolische 13C-Stoffflussanalyse - vom isotopisch stationären zum instationären Fall
With the established tools of $^{13}$C-Metabolic Flux Analysis (MFA) up to now it is not possible to analyse the metabolism of microorganisms in biotechnological production processes because of the long labelling time. Due to the proceeding development in mass spectrometry it is now possible to meas...
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Personal Name(s): | Grönke, Karsten (Corresponding author) |
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Contributing Institute: |
Biotechnologie 2; IBT-2 |
Imprint: |
Jülich
Forschungszentrum Jülich GmbH Zentralbibliothek, Verlag
2010
|
Dissertation Note: |
Universität Siegen, Diss., 2010 |
ISBN: |
978-3-89336-713-9 |
Document Type: |
Book Dissertation / PhD Thesis |
Research Program: |
Biotechnologie |
Series Title: |
Schriften des Forschungszentrums Jülich. Reihe Gesundheit / Health
39 |
Subject (ZB): | |
Link: |
OpenAccess |
Publikationsportal JuSER |
With the established tools of $^{13}$C-Metabolic Flux Analysis (MFA) up to now it is not possible to analyse the metabolism of microorganisms in biotechnological production processes because of the long labelling time. Due to the proceeding development in mass spectrometry it is now possible to measure the $^{13}$C-labelling directly in the intracellular metabolites of the central metabolism (glycolysis, pentose-phosphate-pathway and citric acid cycle). Here it is assumed that an isotopic steady state will be reached in a much shorter time. Main objective of this work was to use this data and reduce the labelling time to make it possible to analyse the cell metabolism in production processes that are mainly batch and fed batch processes. [...] |