This title appears in the Scientific Report :
2013
Please use the identifier:
http://dx.doi.org/10.1117/1.JBO.18.11.111402 in citations.
On-chip optical stimulation and electrical recording from cells
On-chip optical stimulation and electrical recording from cells
We present an optoelectrical device capable of in vitro optical stimulation and electrophysiological recording. The device consists of an array of micropixellated InGaN light-emitting diodes coupled to a custom-made ultrathin planar microelectrode array. Cells can be cultured directly on the chip fo...
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Personal Name(s): | Yakushenko, Alexey |
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Gong, Zheng / Maybeck, Vanessa / Hofmann, Boris / Gu, Erdan / Dawson, Martin / Offenhäusser, Andreas / Wolfrum, Bernhard (Corresponding author) | |
Contributing Institute: |
JARA-FIT; JARA-FIT Bioelektronik; PGI-8 Bioelektronik; ICS-8 |
Published in: | Journal of biomedical optics, 18 (2013) 11, S. 111402 |
Imprint: |
Bellingham, Wash.
SPIE
2013
|
PubMed ID: |
23788259 |
DOI: |
10.1117/1.JBO.18.11.111402 |
Document Type: |
Journal Article |
Research Program: |
Helmholtz Young Investigators Group Physics of the Cell Sensorics and bioinspired systems |
Publikationsportal JuSER |
We present an optoelectrical device capable of in vitro optical stimulation and electrophysiological recording. The device consists of an array of micropixellated InGaN light-emitting diodes coupled to a custom-made ultrathin planar microelectrode array. Cells can be cultured directly on the chip for short- and long-term electrophysiological experiments. To show the functionality of the device, we transfected a cardiomyocyte-like cell line (HL-1) with a light-sensitive protein channelrhodopsin. We monitored action potentials of individual, spontaneously beating, HL-1 cells growing on the chip by extracellular electrical recordings. On-chip optical stimulation was demonstrated by triggering network activity in a confluent HL-1 cell culture and visualized by calcium imaging. We see the potential of our system for electrophysiological experiments with optogenetically modified cells. Optical stimulation can be performed directly on the chip without additional optical components or external light sources. |