This title appears in the Scientific Report :
2011
Please use the identifier:
http://dx.doi.org/10.1016/j.biomaterials.2010.11.036 in citations.
Axon guidance of rat cortical neurons by microcontact printed gradients
Axon guidance of rat cortical neurons by microcontact printed gradients
Substrate-bound gradients expressed in numerous spatio-temporal patterns play a crucial role during the development of complex neural circuits. A deeper understanding of the axon guidance mechanism is provided by studying the effect of a defined substrate-bound cue on a confined neural network. In t...
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Personal Name(s): | Fricke, R. |
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Zentis, P. D. / Rajappa, L. T. / Hofmann, B. / Banzet, M. / Offenhäusser, A. / Meffert, S. H. | |
Contributing Institute: |
Bioelektronik; ICS-8 Bioelektronik; PGI-8 JARA-FIT; JARA-FIT |
Published in: | Biomaterials, 32 (2011) S. 2070 - 2076 |
Published in: |
.. |
Imprint: |
Amsterdam [u.a.]
Elsevier Science
2011
|
Physical Description: |
2070 - 2076 |
PubMed ID: |
21167596 |
DOI: |
10.1016/j.biomaterials.2010.11.036 |
Document Type: |
Journal Article |
Research Program: |
BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung Grundlagen für zukünftige Informationstechnologien |
Series Title: |
Biomaterials
32 |
Subject (ZB): | |
Publikationsportal JuSER |
Substrate-bound gradients expressed in numerous spatio-temporal patterns play a crucial role during the development of complex neural circuits. A deeper understanding of the axon guidance mechanism is provided by studying the effect of a defined substrate-bound cue on a confined neural network. In this study, we constructed a discontinuous substrate-bound gradient to control neuronal cell position, the path of neurite growth, and axon directionality. A variety of gradient patterns, with slight changes in slope, width, and length were designed and fabricated by microcontact printing using laminin/poly-l-lysine (PLL) or PLL alone. The gradients were tested for neurite growth and their impact on axon guidance of embryonic rat cortical neurons. The neurite length was determined and the axon was evaluated by Tau-1 immunostaining. We found that the microgradients of laminin/PLL and PLL directed neurons' adhesion, differentially controlled the neurite growth, and guided up to 84% of the axons. The effect of the protein micropattern on axon guidance and neurite growth depended on the protein and geometric parameters used. Our approach proved to be very successful in guiding axons of single multipolar neurons with very high efficiency. It could thereby be useful to engineer defined neural networks for analyzing signal processing of functional circuits, as well as to unravel fundamental questions of the axon guidance mechanism. |