This title appears in the Scientific Report :
2014
Please use the identifier:
http://dx.doi.org/10.5162/11dss2013/3.4 in citations.
3.4 - Detection of the metabolic activity of cells by differential measurements based on a single light-adressable potentiometric sensor chip
3.4 - Detection of the metabolic activity of cells by differential measurements based on a single light-adressable potentiometric sensor chip
The metabolic activity of Chinese hamster ovary (CHO) cells was successfully observed using a lightaddressable potentiometric sensor (LAPS). The dependency of the different glucose concentrations (17-200 mM) follows a Michaelis-Menten kinetics like trajectory. Moreover, a good adhesion of CHO cells...
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Personal Name(s): | Takenaga, S. (Corresponding author) |
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Herrera, C. / Werner, C. F. / Biselli, M. / Schnitzler, Th. / Schöning, M. J. / Öhlschläger, P. / Wagner, T. | |
Contributing Institute: |
JARA-FIT; JARA-FIT Bioelektronik; PGI-8 |
Imprint: |
AMA Service GmbH, P.O. Box 2352, 31506 Wunstorf, Germany
2013
|
Physical Description: |
63-67 |
DOI: |
10.5162/11dss2013/3.4 |
Conference: | 11. Dresdner Sensor-Symposium, Dresden (Germany), 2013-12-11 - 2013-12-11 |
Document Type: |
Contribution to a conference proceedings |
Research Program: |
Sensorics and bioinspired systems |
Publikationsportal JuSER |
The metabolic activity of Chinese hamster ovary (CHO) cells was successfully observed using a lightaddressable potentiometric sensor (LAPS). The dependency of the different glucose concentrations (17-200 mM) follows a Michaelis-Menten kinetics like trajectory. Moreover, a good adhesion of CHO cells on the Ta2O5 surface of the sensor chip was observed, and the cells could culture under normal conditions. These results demonstrate the detection of the metabolic response from cells by means of a LAPS-based set-up. |