This title appears in the Scientific Report :
2014
Please use the identifier:
http://dx.doi.org/10.1016/j.copbio.2014.02.008 in citations.
Single-cell microfluidics: opportunity for bioprocess development
Single-cell microfluidics: opportunity for bioprocess development
Cell-to-cell heterogeneity in microbial biotechnological processes caused by biological (intrinsic) and environmental (extrinsic) fluctuations can have a severe impact on productivity. However, as yet little is known about the complex interplay between environmental reactor dynamics and cellular act...
Saved in:
Personal Name(s): | Grünberger, Alexander (Corresponding Author) |
---|---|
Wiechert, Wolfgang / Kohlheyer, Dietrich | |
Contributing Institute: |
Biotechnologie; IBG-1 |
Published in: | Current opinion in biotechnology, 29 (2014) S. 15 - 23 |
Imprint: |
Amsterdam [u.a.]
Elsevier Science
2014
|
DOI: |
10.1016/j.copbio.2014.02.008 |
PubMed ID: |
24642389 |
Document Type: |
Journal Article |
Research Program: |
ohne Topic |
Publikationsportal JuSER |
Cell-to-cell heterogeneity in microbial biotechnological processes caused by biological (intrinsic) and environmental (extrinsic) fluctuations can have a severe impact on productivity. However, as yet little is known about the complex interplay between environmental reactor dynamics and cellular activity. A few years ago, innovative microfluidic systems were introduced facilitating the spatiotemporal analysis of single cells under well-defined environmental conditions allowing so far unachievable insights into population heterogeneity and bioreactor inhomogeneity. Examples of microfabricated systems include microfluidic cavities harbouring micropopulations of several thousand cells down to femtolitre-size structures entrapping individual bacteria. In well-defined perfusion experiments, central questions in biotechnology regarding, for example, growth, productivity, and heterogeneity on the single-cell level have been addressed for the first time. Microfluidics will take its place as a single-cell analytical technique in biotechnological process and strain characterization. |