Increased Micronucleus Induction in 170Tm-irradiated Nanogold-labeled SCL II-cells
Increased Micronucleus Induction in 170Tm-irradiated Nanogold-labeled SCL II-cells
Photoelectric absorption of photons with energies slightly above the K shell binding energy of an appropriate element can result in the emission of a shower of lowenergy Auger-electrons. It is well known that those electrons released by Augerelectron-emitting radionuclides located in the immediate v...
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Personal Name(s): | Kriehuber, Ralf (Corresponding author) |
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von Ameln, Marcel / Bahn, S. / Pomplun, Ekkehard | |
Contributing Institute: |
Sicherheit und Strahlenschutz, Umgebungsüberwachung,Strahlenbiologie; S-US |
Published in: | Radioprotection, 43 (2008) 5, S. 271 |
Imprint: |
Les Ulis
EDP Sciences
2008
|
DOI: |
10.1051/radiopro:2008681 |
Conference: | 36th Annual Meeting of the European Radiation Research Society |
Document Type: |
Journal Article |
Research Program: |
ohne Topic |
Subject (ZB): | |
Link: |
OpenAccess OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/16293 in citations.
Photoelectric absorption of photons with energies slightly above the K shell binding energy of an appropriate element can result in the emission of a shower of lowenergy Auger-electrons. It is well known that those electrons released by Augerelectron-emitting radionuclides located in the immediate vicinity of the DNA cause high-LET-type damage and induce an enhanced relative biological effectiveness when compared to low-LET radiation. Therefore, an enhanced biological effectiveness is expected after photon activation as well. To proof if photoelectric absorptionleads to an increased cellular radiotoxicity we investigated in SCL II-cells whether photon activation of intracellular located nano-sized gold particles is feasibleto enhance cyto- and genotoxic effects in vitro. SCL II-cells were transfected with colloidal nano-sized gold particles (40 nm) and gold-labeled DNA-triplexforming-oligonucleotides (TFO) and irradiated with a suitable 170Tm source (micro seeds). Genotoxicity was assessed using the Micronucleus-Assay and cytotoxicity was investigated using the Colony-Forming-Assay. Preliminary results indicate that Nanogold-labeled SCL II-cells show a 2-fold increase in micronucleus formation when compared to irradiated non-labeled cells. Non-irradiated NanogoldlabeledSCL II-cells showed the same background level of micronucleated cells as non-labeled SCLII-cells. The mitotic activity was neither disturbed by the goldlabeling nor the transfection procedure. Cytotoxic effects are less prominent but still need further investigation. Photon activation might be a promising approach to increase the biological effectiveness of low-LET-radiation and might be of great value for new brachytherapy strategies.This work is financially supported by STEP Pockau GmbH |