This title appears in the Scientific Report :
2014
Please use the identifier:
http://dx.doi.org/10.1016/j.copbio.2014.01.005 in citations.
Please use the identifier: http://hdl.handle.net/2128/8018 in citations.
Looking for the pick of the bunch: high-throughput screening of producing microorganisms with biosensors
Looking for the pick of the bunch: high-throughput screening of producing microorganisms with biosensors
The engineering of microbial strains for the production of small molecules of biotechnological interest is a time-consuming, laborious and expensive process. This can be mostly attributed to the fact that good producers cannot be readily obtained by high-throughput screening approaches since increas...
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Personal Name(s): | Schallmey, Marcus |
---|---|
Frunzke, Julia / Eggeling, Lothar / Marienhagen, Jan (Corresponding Author) | |
Contributing Institute: |
Biotechnologie; IBG-1 |
Published in: | Current opinion in biotechnology, 26 (2014) S. 148 - 154 |
Imprint: |
Amsterdam [u.a.]
Elsevier Science
2014
|
DOI: |
10.1016/j.copbio.2014.01.005 |
PubMed ID: |
24480185 |
Document Type: |
Journal Article |
Research Program: |
ohne Topic |
Link: |
OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/8018 in citations.
The engineering of microbial strains for the production of small molecules of biotechnological interest is a time-consuming, laborious and expensive process. This can be mostly attributed to the fact that good producers cannot be readily obtained by high-throughput screening approaches since increased product formation usually does not confer a clear phenotype to producing strain variants. Recently, advances were made in the design and construction of genetically encoded RNA aptamer-based or transcription factor-based biosensors for detecting small molecules at the single-cell level. The first promising examples for the application of these molecular biosensors in combination with fluorescent-activated cell sorting as a high-throughput screening device demonstrated the value and potential of these new tools for microbial strain development. |