This title appears in the Scientific Report :
2011
Please use the identifier:
http://dx.doi.org/10.1021/ja108626w in citations.
Global Structure of Forked DNA in Solution Revealed by High-Resolution Single-Molecule FRET
Global Structure of Forked DNA in Solution Revealed by High-Resolution Single-Molecule FRET
Branched DNA structures play critical roles in DNA replication, repair, and recombination in addition to being key building blocks for DNA nanotechnology. Here we combine single-molecule multiparameter fluorescence detection and molecular dynamics simulations to give a general approach to global str...
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Personal Name(s): | Sabir, T. |
---|---|
Schröder, G.F. / Toulmin, A. / McGlynn, P. / Magennis, S.W. | |
Contributing Institute: |
Strukturbiochemie; ICS-6 |
Published in: | Journal of the American Chemical Society, 133 (2011) S. 1188 - 1191 |
Imprint: |
Washington, DC
American Chemical Society
2011
|
Physical Description: |
1188 - 1191 |
PubMed ID: |
21174398 |
DOI: |
10.1021/ja108626w |
Document Type: |
Journal Article |
Research Program: |
BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung Funktion und Dysfunktion des Nervensystems |
Series Title: |
Journal of the American Chemical Society
133 |
Subject (ZB): | |
Publikationsportal JuSER |
Branched DNA structures play critical roles in DNA replication, repair, and recombination in addition to being key building blocks for DNA nanotechnology. Here we combine single-molecule multiparameter fluorescence detection and molecular dynamics simulations to give a general approach to global structure determination of branched DNA in solution. We reveal an open, planar structure of a forked DNA molecule with three duplex arms and demonstrate an ion-induced conformational change. This structure will serve as a benchmark for DNA-protein interaction studies. |