This title appears in the Scientific Report :
2011
Please use the identifier:
http://hdl.handle.net/2128/4698 in citations.
High Temperature Radio-Frequency Superconducting Quantum Interference Device System for Detection of Magnetic Nanoparticles
High Temperature Radio-Frequency Superconducting Quantum Interference Device System for Detection of Magnetic Nanoparticles
Ths doctoral thesis was aimed at establishing a set-up with high-temperature superconductor (HTS) radio-frequency (rf) superconducting quantum interference device (SQUID) technology for the detection of magnetic nanoparticle immunoassays It was part of the EU-project "Biodiagnostics" runni...
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Personal Name(s): | Pretzell, Alf (Corresponding author) |
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Contributing Institute: |
JARA-FIT; JARA-FIT Bioelektronik; PGI-8 |
Imprint: |
Jülich
Forschungszentrum Jülich GmbH Zentralbibliothek, Verlag
2011
|
Dissertation Note: |
RWTH Aachen, Diss., 2011 |
ISBN: |
978-3-89336-814-3 |
Document Type: |
Book Dissertation / PhD Thesis |
Research Program: |
Grundlagen für zukünftige Informationstechnologien |
Series Title: |
Schriften des Forschungszentrums Jülich. Schlüsseltechnologien / Key Technologies
48 |
Subject (ZB): | |
Link: |
OpenAccess |
Publikationsportal JuSER |
Ths doctoral thesis was aimed at establishing a set-up with high-temperature superconductor (HTS) radio-frequency (rf) superconducting quantum interference device (SQUID) technology for the detection of magnetic nanoparticle immunoassays It was part of the EU-project "Biodiagnostics" running from 2005 to 2008. The method of magnetic binding assays was developed as an alternative to other methods of concentration determination like enzyme linked immunosorbent assay (ELISA), of fluorescent immunoassay. The ELISA has sensitivities down to analyte-concentrations of pg/ml p. 261 in [RAEM 2007]. Multiple incubation and washing steps have to be performed for these techniques, the analyte has to dffuse to the site of binding. The magnetic assay uses magnetic nanoparticles as markers for the substance to be detected. It is being explored by current research and shows similar sensitivity [Enpuku 2007] compared to Elisa but in contrast $\bullet$ does not need any washing and can be read out directly after binding $\bullet$ can be applied insolution with opaque media, e. g. blood or muddy water $\bullet$ additionally allows magnetic separation of concentration $\bullet$ in combination with small magnetoresistive of Hall sensors, allows detection of only a few particles or even single beads. - For medical or enironmental samples, maybe opaque and containing a multitide of substances, it would be advantageous to devide an instrument, which allows to be read out quickly and with high sensitivity Due to the mentioned items the magnetic assay might be a possibility here. Magnetic colloids have been investigated since the middle of the sixties when it became feasible to synthesize them - p. 1 in [Blum 1997]. As a possible application for these colloids Kriz introduced the idea of magnetic immunoassays in 1996 [Kriz 1996]. |