This title appears in the Scientific Report :
2014
Please use the identifier:
http://dx.doi.org/10.1111/mmi.12633 in citations.
Phosphatase activity of the histidine kinases ensures pathway specificity of the ChrSA and HrrSA two-component systems in C orynebacterium glutamicum
Phosphatase activity of the histidine kinases ensures pathway specificity of the ChrSA and HrrSA two-component systems in C orynebacterium glutamicum
The majority of bacterial genomes encode a high number of two-component systems controlling gene expression in response to a variety of different stimuli. The Gram-positive soil bacterium Corynebacterium glutamicum contains two homologous two-component systems (TCS) involved in the haem-dependent re...
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Personal Name(s): | Hentschel, Eva (Corresponding Author) |
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Mack, Christina / Gätgens, Cornelia / Bott, Michael / Brocker, Melanie / Frunzke, Julia | |
Contributing Institute: |
Biotechnologie; IBG-1 |
Published in: | Molecular microbiology, 92 (2014) 6, S. 1326 - 1342 |
Imprint: |
Oxford [u.a.]
Wiley-Blackwell
2014
|
DOI: |
10.1111/mmi.12633 |
Document Type: |
Journal Article |
Research Program: |
ohne Topic |
Publikationsportal JuSER |
The majority of bacterial genomes encode a high number of two-component systems controlling gene expression in response to a variety of different stimuli. The Gram-positive soil bacterium Corynebacterium glutamicum contains two homologous two-component systems (TCS) involved in the haem-dependent regulation of gene expression. Whereas the HrrSA system is crucial for utilization of haem as an alternative iron source, ChrSA is required to cope with high toxic haem levels. In this study, we analysed the interaction of HrrSA and ChrSA in C. glutamicum. Growth of TCS mutant strains, in vitro phosphorylation assays and promoter assays of PhrtBA and PhmuO fused to eyfp revealed cross-talk between both systems. Our studies further indicated that both kinases exhibit a dual function as kinase and phosphatase. Mutation of the conserved glutamine residue in the putative phosphatase motif DxxxQ of HrrS and ChrS resulted in a significantly increased activity of their respective target promoters (PhmuO and PhrtBA respectively). Remarkably, phosphatase activity of both kinases was shown to be specific only for their cognate response regulators. Altogether our data suggest the phosphatase activity of HrrS and ChrS as key mechanism to ensure pathway specificity and insulation of these two homologous systems. |