This title appears in the Scientific Report :
2014
Please use the identifier:
http://dx.doi.org/10.1186/scrt500 in citations.
Please use the identifier: http://hdl.handle.net/2128/8034 in citations.
Aromatic-turmerone induces neural stem cell proliferation in vitro and in vivo.
Aromatic-turmerone induces neural stem cell proliferation in vitro and in vivo.
IntroductionAromatic (ar-) turmerone is a major bioactive compound of the herb Curcuma longa. It has been suggested that ar-turmerone inhibits microglia activation, a property that may be useful in treating neurodegenerative disease. Furthermore, the effects of ar-turmerone on neural stem cells (NSC...
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Personal Name(s): | Hucklenbroich, J. (Corresponding Author) |
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Klein, R. / Neumaier, B. / Graf, R. / Fink, G. R. / Schroeter, M. / Rueger, M. A. | |
Contributing Institute: |
Kognitive Neurowissenschaften; INM-3 |
Published in: | Stem cell research & therapy, 5 (2014) 4, S. Article 100 (1-9) |
Imprint: |
London
BioMed Central
2014
|
PubMed ID: |
25928248 |
DOI: |
10.1186/scrt500 |
Document Type: |
Journal Article |
Research Program: |
(Dys-)function and Plasticity Pathophysiological Mechanisms of Neurological and Psychiatric Diseases |
Link: |
OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/8034 in citations.
IntroductionAromatic (ar-) turmerone is a major bioactive compound of the herb Curcuma longa. It has been suggested that ar-turmerone inhibits microglia activation, a property that may be useful in treating neurodegenerative disease. Furthermore, the effects of ar-turmerone on neural stem cells (NSCs) remain to be investigated.MethodsWe exposed primary fetal rat NSCs to various concentrations of ar-turmerone. Thereafter, cell proliferation and differentiation potential were assessed. In vivo, naïve rats were treated with a single intracerebroventricular (i.c.v.) injection of ar-turmerone. Proliferative activity of endogenous NSCs was assessed in vivo, by using noninvasive positron emission tomography (PET) imaging and the tracer [18F]-fluoro-L-thymidine ([18F]FLT), as well as ex vivo.ResultsIn vitro, ar-turmerone increased dose-dependently the number of cultured NSCs, because of an increase in NSC proliferation (P < 0.01). Proliferation data were supported by qPCR-data for Ki-67 mRNA. In vitro as well as in vivo, ar-turmerone promoted neuronal differentiation of NSCs. In vivo, after i.c.v. injection of ar-turmerone, proliferating NSCs were mobilized from the subventricular zone (SVZ) and the hippocampus of adult rats, as demonstrated by both [18F]FLT-PET and histology (P < 0.05).ConclusionsBoth in vitro and in vivo data suggest that ar-turmerone induces NSC proliferation. Ar-turmerone thus constitutes a promising candidate to support regeneration in neurologic disease. |