This title appears in the Scientific Report :
2011
Please use the identifier:
http://dx.doi.org/10.1007/s00253-011-3374-4 in citations.
Increased NADPH availability in Escherichia coli: improvement of the product per glucose ratio in reductive whole-cell biotransformation
Increased NADPH availability in Escherichia coli: improvement of the product per glucose ratio in reductive whole-cell biotransformation
A basic requirement for the efficiency of reductive whole-cell biotransformations is the reducing capacity of the host. Here, the pentose phosphate pathway (PPP) was applied for NADPH regeneration with glucose as the electron-donating co-substrate using Escherichia coli as host. Reduction of the pro...
Saved in:
Personal Name(s): | Siedler, S. |
---|---|
Bringer, S. / Bott, M. | |
Contributing Institute: |
Biotechnologie 1; IBT-1 |
Published in: | Applied Microbiology and Biotechnology, 92 (2011) S. 929 - 937 |
Imprint: |
Berlin
Springer
2011
|
Physical Description: |
929 - 937 |
PubMed ID: |
21670981 |
DOI: |
10.1007/s00253-011-3374-4 |
Document Type: |
Journal Article |
Research Program: |
Biotechnologie |
Series Title: |
Applied Microbiology and Biotechnology
92 |
Subject (ZB): | |
Publikationsportal JuSER |
A basic requirement for the efficiency of reductive whole-cell biotransformations is the reducing capacity of the host. Here, the pentose phosphate pathway (PPP) was applied for NADPH regeneration with glucose as the electron-donating co-substrate using Escherichia coli as host. Reduction of the prochiral β-keto ester methyl acetoacetate to the chiral hydroxy ester (R)-methyl 3-hydroxybutyrate (MHB) served as a model reaction, catalyzed by an R-specific alcohol dehydrogenase. The main focus was maximization of the reduced product per glucose yield of this pathway-coupled cofactor regeneration with resting cells. With a strain lacking the phosphoglucose isomerase, the yield of the reference strain was increased from 2.44 to 3.78 mol MHB/mol glucose. Even higher yields were obtained with strains lacking either phosphofructokinase I (4.79 mol MHB/mol glucose) or phosphofructokinase I and II (5.46 mol MHB/mol glucose). These results persuasively demonstrate the potential of NADPH generation by the PPP in whole-cell biotransformations. |