This title appears in the Scientific Report :
2014
Please use the identifier:
http://hdl.handle.net/2128/15942 in citations.
Please use the identifier: http://dx.doi.org/10.1016/j.bios.2014.01.034 in citations.
Bioelectrochemical systems with oleylamine-stabilized gold nanostructures and horseradish peroxidase for hydrogen peroxide sensor
Bioelectrochemical systems with oleylamine-stabilized gold nanostructures and horseradish peroxidase for hydrogen peroxide sensor
This paper describes ultrathin gold nanowires (NWs) and nanoparticles (NPs) prepared by oleylamine (OA) synthesis and their assembly with horseradish peroxidase enzyme (HRP) for bioelectrochemical sensing of hydrogen peroxide for the first time. The immobilization of oxidoreductase enzyme HRP on the...
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Personal Name(s): | Koposova, Ekaterina (Corresponding Author) |
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Liu, Xiao / Kisner, A. / Ermolenko, Yury / Shumilova, Galina / Offenhäusser, Andreas / Mourzina, Youlia | |
Contributing Institute: |
JARA-FIT; JARA-FIT Bioelektronik; PGI-8 |
Published in: | Biosensors and bioelectronics, 57 (2014) S. 54 - 58 |
Imprint: |
Amsterdam [u.a.]
Elsevier Science
2014
|
DOI: |
10.1016/j.bios.2014.01.034 |
PubMed ID: |
24534581 |
Document Type: |
Journal Article |
Research Program: |
Sensorics and bioinspired systems |
Link: |
Restricted OpenAccess OpenAccess Restricted OpenAccess OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://dx.doi.org/10.1016/j.bios.2014.01.034 in citations.
This paper describes ultrathin gold nanowires (NWs) and nanoparticles (NPs) prepared by oleylamine (OA) synthesis and their assembly with horseradish peroxidase enzyme (HRP) for bioelectrochemical sensing of hydrogen peroxide for the first time. The immobilization of oxidoreductase enzyme HRP on the electrodes modified with OA gold nanostructures (OANSs) is discussed. The HRP-sensor characteristics, namely sensitivity, working concentration range, sensor-to-sensor and measurement-to-measurement reproducibility as well as long-term stability, are improved significantly compared to the planar thin-film sensors by using OANSs. The thin-film gold electrodes modified with OANWs and OANPs exhibit a catalytic activity towards oxidation of hydrogen peroxide with a working concentration range from 20 µM to 500 µM, a sensitivity of 0.031 A M−1 cm−2 (RSD 0.046) and 0.027 A M−1 cm−2 (RSD 0.045), and a detection limit of 5 µM and 8 µM, respectively (RSD near the detection limits was 9–12%). Our study shows that ultrathin gold nanowires and nanoparticles prepared by oleylamine synthesis are prospective materials to assemble biomolecules into functional nanoarchitectures for enzyme-based bioelectrochemical sensors, metalloprotein bioelectronics, and energy research. |