This title appears in the Scientific Report :
2014
Please use the identifier:
http://dx.doi.org/10.1002/pssa.201330464 in citations.
Toward multi-analyte bioarray sensors: LAPS-based on-chip determination of a Michaelis-Menten-like kinetics for cell culturing
Toward multi-analyte bioarray sensors: LAPS-based on-chip determination of a Michaelis-Menten-like kinetics for cell culturing
The metabolic activity of Chinese hamster ovary (CHO) cells was observed using a light-addressable potentiometric sensor (LAPS). The dependency toward different glucose concentrations (17–200 mM) follows a Michaelis–Menten kinetics trajectory with Km = 32.8 mM, and the obtained Km value in this expe...
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Personal Name(s): | Takenaga, S. |
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Herrera, C. / Werner, C. F. / Biselli, M. / Thönnessen, V. / Schnitzler, Th. / Öhlschläger, P. / Almajhdi, F. N. / Wagner, T. (Corresponding Author) / Schöning, M. J. | |
Contributing Institute: |
JARA-FIT; JARA-FIT Bioelektronik; PGI-8 |
Published in: | Physica status solidi / A, 211 (2014) 6, S. 1410 - 1415 |
Imprint: |
Weinheim
Wiley-VCH
2014
|
DOI: |
10.1002/pssa.201330464 |
Document Type: |
Journal Article |
Research Program: |
Sensorics and bioinspired systems |
Publikationsportal JuSER |
The metabolic activity of Chinese hamster ovary (CHO) cells was observed using a light-addressable potentiometric sensor (LAPS). The dependency toward different glucose concentrations (17–200 mM) follows a Michaelis–Menten kinetics trajectory with Km = 32.8 mM, and the obtained Km value in this experiment was compared with that found in literature. In addition, the pH shift induced by glucose metabolism of tumor cells transfected with the HPV-16 genome (C3 cells) was successfully observed. These results indicate the possibility to determine the tumor cells metabolism with a LAPS-based measurement device. |