This title appears in the Scientific Report :
2015
Please use the identifier:
http://dx.doi.org/10.1021/acs.jpcb.5b02170 in citations.
Accurate Fluorescence Quantum Yield Determination by Fluorescence Correlation Spectroscopy
Accurate Fluorescence Quantum Yield Determination by Fluorescence Correlation Spectroscopy
Here, we present a comparative method for the accurate determination of fluorescence quantum yields (QYs) by fluorescence correlation spectroscopy. By exploiting the high sensitivity of single-molecule spectroscopy, we obtain the QYs of samples in the microliter range and at (sub)nanomolar concentra...
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Personal Name(s): | Kempe, Daryan |
---|---|
Schöne, Antonie / Fitter, Jörg (Corresponding Author) / Gabba, Matteo (Corresponding Author) | |
Contributing Institute: |
Molekulare Biophysik; ICS-5 |
Published in: |
The @journal of physical chemistry |
Imprint: |
Washington, DC
Soc.
2015
|
DOI: |
10.1021/acs.jpcb.5b02170 |
Document Type: |
Journal Article |
Research Program: |
Functional Macromolecules and Complexes |
Publikationsportal JuSER |
Here, we present a comparative method for the accurate determination of fluorescence quantum yields (QYs) by fluorescence correlation spectroscopy. By exploiting the high sensitivity of single-molecule spectroscopy, we obtain the QYs of samples in the microliter range and at (sub)nanomolar concentrations. Additionally, in combination with fluorescence lifetime measurements, our method allows the quantification of both static and collisional quenching constants. Thus, besides being simple and fast, our method opens up the possibility to photophysically characterize labeled biomolecules under application-relevant conditions and with low sample consumption, which is often important in single-molecule studies. |