This title appears in the Scientific Report :
2015
Please use the identifier:
http://dx.doi.org/10.1038/nsmb.3002 in citations.
Crystal structure of a light-driven sodium pump
Crystal structure of a light-driven sodium pump
Recently, the first known light-driven sodium pumps, from the microbial rhodopsin family, were discovered. We have solved the structure of one of them, Krokinobacter eikastus rhodopsin 2 (KR2), in the monomeric blue state and in two pentameric red states, at resolutions of 1.45 Å and 2.2 and 2.8 Å...
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Personal Name(s): | Gushchin, Ivan |
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Shevchenko, Vitaly / Polovinkin, V. / Kovalev, Kirill / Alekseev, Alexey / Round, E. / Borshchevskiy, Valentin / Balandin, Taras / Popov, A. / Gensch, Thomas / Fahlke, Christoph / Bamann, C. / Willbold, Dieter / Büldt, Georg / Bamberg, E. / Gordeliy, Valentin (Corresponding Author) | |
Contributing Institute: |
Zelluläre Biophysik; ICS-4 Strukturbiochemie; ICS-6 Molekulare Biophysik; ICS-5 |
Published in: | Nature Structural & Molecular Biology Nature structural & molecular biology, 22 22 (2015 2015) 5 5, S. 390-395 390-395 |
Imprint: |
New York, NY
Nature America
2015
|
DOI: |
10.1038/nsmb.3002 |
PubMed ID: |
25849142 |
Document Type: |
Journal Article |
Research Program: |
Engineering Cell Function |
Publikationsportal JuSER |
Recently, the first known light-driven sodium pumps, from the microbial rhodopsin family, were discovered. We have solved the structure of one of them, Krokinobacter eikastus rhodopsin 2 (KR2), in the monomeric blue state and in two pentameric red states, at resolutions of 1.45 Å and 2.2 and 2.8 Å, respectively. The structures reveal the ion-translocation pathway and show that the sodium ion is bound outside the protein at the oligomerization interface, that the ion-release cavity is capped by a unique N-terminal α-helix and that the ion-uptake cavity is unexpectedly large and open to the surface. Obstruction of the cavity with the mutation G263F imparts KR2 with the ability to pump potassium. These results pave the way for the understanding and rational design of cation pumps with new specific properties valuable for optogenetics. |