This title appears in the Scientific Report :
2015
Please use the identifier:
http://dx.doi.org/10.1063/1.4928322 in citations.
Please use the identifier: http://hdl.handle.net/2128/17242 in citations.
Characterization of the mechanical properties of HL-1 cardiomyocytes with high throughput magnetic tweezers
Characterization of the mechanical properties of HL-1 cardiomyocytes with high throughput magnetic tweezers
We characterized the mechanical properties of cardiomyocyte-like HL-1 cells using our recently developed multi-pole magnetic tweezers. With the optimized design, both high force and high throughput are achieved at the same time. Force up to 100 pN can be applied on a 1 μm diameter superparamagnetic...
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Personal Name(s): | Chen, La |
---|---|
Maybeck, Vanessa / Offenhäusser, Andreas / Krause, Hans-Joachim (Corresponding author) | |
Contributing Institute: |
JARA-FIT; JARA-FIT Bioelektronik; ICS-8 Bioelektronik; PGI-8 |
Published in: | Applied physics letters, 107 (2015) 5, S. 053703 - |
Imprint: |
Melville, NY
American Inst. of Physics
2015
|
DOI: |
10.1063/1.4928322 |
Document Type: |
Journal Article |
Research Program: |
Physical Basis of Diseases Engineering Cell Function |
Link: |
OpenAccess OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/17242 in citations.
We characterized the mechanical properties of cardiomyocyte-like HL-1 cells using our recently developed multi-pole magnetic tweezers. With the optimized design, both high force and high throughput are achieved at the same time. Force up to 100 pN can be applied on a 1 μm diameter superparamagnetic bead in a workspace with 60 μm radius, which is encircled symmetrically by 3 sharp magnetic tips. By adjusting the coil currents, both the strength and direction of force can be controlled. The result shows that both viscosity and shear elastic modulus of HL-1 cells exhibit an approximately log-normal distribution. The cells became stiffer as they matured, consistent with a transition from proliferating cells to contractile muscle tissue. Moreover, the mechanical properties of HL-1 cells show high heterogeneity, which agrees well with their physiological structure. |