This title appears in the Scientific Report :
2012
Please use the identifier:
http://dx.doi.org/10.1007/s10735-012-9395-1 in citations.
Locust primary neuronal culture for the study of synaptic transmission
Locust primary neuronal culture for the study of synaptic transmission
We have designed a cell culture system for thoracic neurons of adult Locusta migratoria that enables the establishment of functional synapses in vitro. Patch-clamp recordings revealed three different neuron classes. About half of the neurons (47%) had unexcitable somata with outward and no inward co...
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Personal Name(s): | Weigel, S. |
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Schulte, P. / Meffert, S. / Bräunig, P. / Offenhäusser, A. | |
Contributing Institute: |
Bioelektronik; ICS-8 Bioelektronik; PGI-8 JARA-FIT; JARA-FIT |
Published in: | Journal of molecular histology, 43 (2012) S. 405 - 419 |
Imprint: |
Dordrecht [u.a.]
Springer Science + Business Media B.V.
2012
|
Physical Description: |
405 - 419 |
PubMed ID: |
22403023 |
DOI: |
10.1007/s10735-012-9395-1 |
Document Type: |
Journal Article |
Research Program: |
Grundlagen für zukünftige Informationstechnologien |
Series Title: |
Journal of Molecular Histology
43 |
Subject (ZB): | |
Publikationsportal JuSER |
We have designed a cell culture system for thoracic neurons of adult Locusta migratoria that enables the establishment of functional synapses in vitro. Patch-clamp recordings revealed three different neuron classes. About half of the neurons (47%) had unexcitable somata with outward and no inward conductance. The other half generated either single (37%) or multiple action potentials (18%) and differed mainly in lower outward conductance. Selectively stained motor neurons were analyzed to demonstrate varied physiological properties due to culture conditions. Using paired patch clamp recordings we demonstrate directly synaptic transmission in morphologically connected neurons in vitro. Presynaptic stimulation resulted in postsynaptic potentials in 42 pairs of neurons tested, independent of the type of neuron. According to pharmacological experiments most of these synapses were either glutamatergic or GABAergic. In addition to these chemical synapses, electrical synapses were found. With the demonstration of synapse formation in cell culture of adult locust neurons, this study provides the basis for the future analysis of more defined insect neuronal circuits in culture. |