This title appears in the Scientific Report :
2012
Please use the identifier:
http://dx.doi.org/10.1002/pssa.201100801 in citations.
Nutrient concentration-sensitive microorganism-based biosensor
Nutrient concentration-sensitive microorganism-based biosensor
This work presents a biosensor based on living cells immobilised on a light-addressable potentiometric sensor (LAPS). The set-up is sensitive to the concentrations of nutrients metabolised by bacteria, which results in an extracellular acidification. This is exemplarily demonstrated with glucose as...
Saved in:
Personal Name(s): | Werner, C. F. |
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Gröbel, S. / Krumbe, C. / Wagner, T. / Selmer, T. / Yoshinobu, T. / Baumann, M. E. M. / Keusgen, M. / Schöning, M. J. | |
Contributing Institute: |
Bioelektronik; PGI-8 JARA-FIT; JARA-FIT |
Published in: | Physica status solidi / A, 209 (2012) 5, S. 900 - 904 |
Imprint: |
Weinheim
Wiley-VCH
2012
|
Physical Description: |
900 - 904 |
DOI: |
10.1002/pssa.201100801 |
Document Type: |
Journal Article |
Research Program: |
Grundlagen für zukünftige Informationstechnologien |
Series Title: |
Physica Status Solidi A
209 |
Subject (ZB): | |
Publikationsportal JuSER |
This work presents a biosensor based on living cells immobilised on a light-addressable potentiometric sensor (LAPS). The set-up is sensitive to the concentrations of nutrients metabolised by bacteria, which results in an extracellular acidification. This is exemplarily demonstrated with glucose as nutrient and Escherichia coli as microorganism. The function of this biosensor is comparable to an enzyme-based field-effect sensor when using diffusion limitation. With the help of addressability of the LAPS an on-chip differential set-up was developed. Thus, external influences such as sensor drift, temperature and external pH changes can be minimised. (c) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim |