This title appears in the Scientific Report :
2000
Fed-batch production of recombinant human calcitonin precursor fusion-protein using Staphylococcus carnosus as expression-secretion system
Fed-batch production of recombinant human calcitonin precursor fusion-protein using Staphylococcus carnosus as expression-secretion system
A pH-auxostatic fed-batch process was developed for the secretory production of a fusion protein consisting of the pro-part of Staphylococcus hyicus lipase and two synthetic human calcitonin (hCT) precursor repeats under the control of a xylose-inducible promotor from Staphylococcus xylosus. Using g...
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Personal Name(s): | Dilsen, S. |
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Paul, W. / Sandgathe, A. / Tippe, D. / Freudl, R. / Thömmes, J. / Kula, M. R. / Takors, R. / Wandrey, C. / Weuster-Botz, D. | |
Contributing Institute: |
Institut für Biotechnologie; IBT |
Published in: | Applied microbiology and biotechnology, 54 (2000) S. 361 - 369 |
Imprint: |
Berlin
Springer
2000
|
Physical Description: |
361 - 369 |
Document Type: |
Journal Article |
Research Program: |
Mikrobiologische Grundlagen der Gewinnung von Proteinen Verfahrenstechnik zur mikrobiellen Gewinnung von Primärmetaboliten |
Series Title: |
Applied Microbiology and Biotechnology
54 |
Subject (ZB): | |
Publikationsportal JuSER |
A pH-auxostatic fed-batch process was developed for the secretory production of a fusion protein consisting of the pro-part of Staphylococcus hyicus lipase and two synthetic human calcitonin (hCT) precursor repeats under the control of a xylose-inducible promotor from Staphylococcus xylosus. Using glycerol as the energy source and pH-controlled addition of yeast extract resulted in the production of 2000 mg l(-1) of the fusion protein (420 mg l(-1) of the recombinant hCT precursor) within 14 h, reaching 45 g l(-1) cell dry mass with Staphylococcus carnosus in a stirred-tank reactor. Product titer and space-time yield (30 mg calcitonin precursor l(-1) h(-1)) were thus improved by a factor of 2, and 4.5, respectively, compared to Escherichia coli expression-secretion systems for the production of calcitonin precursors. Two hundred grams of the fusion protein was secreted by the recombinant S. carnosus on a 150-1 scale (scale-up factor of 50) with a minimum use of technical-grade yeast extract (40 mg fusion protein g(-1) yeast extract). |