This title appears in the Scientific Report :
2001
Please use the identifier:
http://dx.doi.org/10.1006/mben.2001.0198 in citations.
Metabolic consequences of altered phosphoenolpyruvate carboxykinase activity in Corynebacterium glutamicum reveal anaplerotic regulation mechanisms in vivo
Metabolic consequences of altered phosphoenolpyruvate carboxykinase activity in Corynebacterium glutamicum reveal anaplerotic regulation mechanisms in vivo
Corynebacterium glutamicum possesses high in vivo activity of the gluconeogenic phosphoenolpyruvate carboxykinase (PEPCk) during growth on glucose, resulting together with anaplerotic carboxylation reactions in a PEP/pyruvate/oxaloacetate substrate cycle. The present study investigated the changes i...
Saved in:
Personal Name(s): | Petersen, S. |
---|---|
Mack, C. / de Graaf, A. A. / Riedel, C. / Eikmanns, B. J. / Sahm, H. | |
Contributing Institute: |
Biotechnologie 1; IBT-1 |
Published in: | Metabolic engineering, 3 (2001) S. 344 - 361 |
Imprint: |
Orlando, Fla.
Academic Press
2001
|
Physical Description: |
344 - 361 |
PubMed ID: |
11676569 |
DOI: |
10.1006/mben.2001.0198 |
Document Type: |
Journal Article |
Research Program: |
Entwicklung von Mikroorganismen für die Herstellung von Primärmetaboliten |
Series Title: |
Metabolic Engineering
3 |
Subject (ZB): | |
Publikationsportal JuSER |
Corynebacterium glutamicum possesses high in vivo activity of the gluconeogenic phosphoenolpyruvate carboxykinase (PEPCk) during growth on glucose, resulting together with anaplerotic carboxylation reactions in a PEP/pyruvate/oxaloacetate substrate cycle. The present study investigated the changes in intracellular fluxes and metabolite concentrations that are caused by altered PEPCk activity in L-lysine-producing C. glutamicum MH20-22B, applying a recently developed (13)C labeling-based strategy for anaplerotic flux resolution and quantification. Abolition of PEPCk activity by deletion of the respective pck gene resulted in increased intracellular concentrations of oxaloacetate L-aspartate, alpha-ketoglutarate, pyruvate, and L-lysine and in a 60% enhanced flux toward L-lysine biosynthesis, whereas increasing the PEPCk activity by pck overexpression had opposite effects. The results of the combined measurements of enzyme activities, in vivo fluxes, and metabolite concentrations were exploited to elucidate the in vivo regulation of anaplerotic reactions in C. glutamicum, and implications for the metabolic engineering of amino-acid-producing strains are discussed. |