This title appears in the Scientific Report :
2016
Please use the identifier:
http://dx.doi.org/10.1002/cctc.201501099 in citations.
Modularized Biocatalysis: Immobilization of Whole Cells for Preparative Applications in Microaqueous Organic Solvents
Modularized Biocatalysis: Immobilization of Whole Cells for Preparative Applications in Microaqueous Organic Solvents
The use of whole-cell biocatalysts enables catalyst application in microaqueous reaction systems, in which the liquid phase consists of high substrate loadings in organic solvents, to enable access to high concentrations of easy-to-purify product. One current research focus is the modularization of...
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Personal Name(s): | Wachtmeister, Jochen |
---|---|
Mennicken, Philip / Hunold, Andreas / Rother, Dörte (Corresponding author) | |
Contributing Institute: |
Biotechnologie; IBG-1 |
Published in: | ChemCatChem, 8 (2016) 3, S. 607-614 |
Imprint: |
Weinheim
WILEY-VCH Verlag
2016
|
DOI: |
10.1002/cctc.201501099 |
Document Type: |
Journal Article |
Research Program: |
Biotechnology |
Publikationsportal JuSER |
The use of whole-cell biocatalysts enables catalyst application in microaqueous reaction systems, in which the liquid phase consists of high substrate loadings in organic solvents, to enable access to high concentrations of easy-to-purify product. One current research focus is the modularization of single reaction steps to (i) enable flexible combinations into multi-step enzyme reactions, (ii) investigate ideal reaction conditions, and (iii) facilitate catalyst handling and recycling. Therefore, we published the easy-to-apply encapsulation of a lyophilized whole-cell catalyst in a polymeric membrane recently. These catalytic “teabags” were demonstrated to enable flexible catalyst combinations for multi-step reactions and excellent recyclability during repeated batch experiments. We now describe the applicability of these “teabags” on a larger scale by using the new SpinChem reactor and a classical stirred-tank reactor model. As an alternative, we investigate the described alginate entrapment approach and compare the results. The carboligation reaction towards (R)-benzoin, using lyophilized E. coli that enclose Pseudomonas fluorescens benzaldehyde lyase (EC 4.1.2.38), served as a model reaction. It was demonstrated that the catalytic “teabags” are scalable and perform equally on the investigatory 5 mL scale and the preparative 140 mL reactor scale. Tested in a more advanced application, the “teabags” were proven to be useful in a one-pot two-step reaction for the gram-scale production of 1-phenylpropane-1,2-diol by using the SpinChem reactor, which allowed to reach an industrially relevant product concentration (32.9 g L−1) and space–time yield (8.2 g L−1 d−1). |