This title appears in the Scientific Report :
2016
Please use the identifier:
http://dx.doi.org/10.1016/j.bpj.2016.06.003 in citations.
Clathrin assembly regulated by adaptor proteins in coarse-grained models
Clathrin assembly regulated by adaptor proteins in coarse-grained models
The assembly of clathrin triskelia into polyhedral cages during endocytosis is regulated by adaptor proteins (APs). We explore how APs achieve this by developing coarse-grained models for clathrin and AP2, employing a Monte Carlo click interaction, to simulate their collective aggregation behavior....
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Personal Name(s): | Giani, M. |
---|---|
den Otter, W. K. (Corresponding author) / Briels, Willem | |
Contributing Institute: |
Weiche Materie; ICS-3 |
Published in: | Biophysical journal, 111 (2016) 1, S. 222-235 |
Imprint: |
Cambridge, Mass.
Cell Press
2016
|
DOI: |
10.1016/j.bpj.2016.06.003 |
Document Type: |
Journal Article |
Research Program: |
Functional Macromolecules and Complexes |
Publikationsportal JuSER |
The assembly of clathrin triskelia into polyhedral cages during endocytosis is regulated by adaptor proteins (APs). We explore how APs achieve this by developing coarse-grained models for clathrin and AP2, employing a Monte Carlo click interaction, to simulate their collective aggregation behavior. The phase diagrams indicate that a crucial role is played by the mechanical properties of the disordered linker segment of AP. We also present a statistical-mechanical theory for the assembly behavior of clathrin, yielding good agreement with our simulations and experimental data from the literature. Adaptor proteins are found to regulate the formation of clathrin coats under certain conditions, but can also suppress the formation of cages. |