This title appears in the Scientific Report :
2015
Please use the identifier:
http://hdl.handle.net/2128/9812 in citations.
Please use the identifier: http://dx.doi.org/10.7554/eLife.07624 in citations.
A K + -selective CNG channel orchestrates Ca2 + signalling in zebrafish sperm
A K + -selective CNG channel orchestrates Ca2 + signalling in zebrafish sperm
Calcium in the flagellum controls sperm navigation. In sperm of marine invertebrates and mammals, Ca2+ signalling has been intensely studied, whereas for fish little is known. In sea urchin sperm, a cyclic nucleotide-gated K+ channel (CNGK) mediates a cGMP-induced hyperpolarization that evokes Ca2+...
Saved in:
Personal Name(s): | Fechner, Sylvia |
---|---|
Alvarez, Luis / Bönigk, Wolfgang / Müller, Astrid / Berger, Thomas / Pascal, Rene / Trötschel, Christian / Poetsch, Ansgar / Stölting, Gabriel / Siegfried, Kellee R / Kremmer, Elisabeth / Seifert, Reinhard / Kaupp, U Benjamin (Corresponding author) | |
Contributing Institute: |
Zelluläre Biophysik; ICS-4 |
Published in: | eLife, 4 (2015) S. e07624 |
Imprint: |
Cambridge
eLife Sciences Publications
2015
|
DOI: |
10.7554/eLife.07624 |
PubMed ID: |
26650356 |
Document Type: |
Journal Article |
Research Program: |
Engineering Cell Function |
Link: |
OpenAccess OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://dx.doi.org/10.7554/eLife.07624 in citations.
Calcium in the flagellum controls sperm navigation. In sperm of marine invertebrates and mammals, Ca2+ signalling has been intensely studied, whereas for fish little is known. In sea urchin sperm, a cyclic nucleotide-gated K+ channel (CNGK) mediates a cGMP-induced hyperpolarization that evokes Ca2+ influx. Here, we identify in sperm of the freshwater fish Danio rerio a novel CNGK family member featuring non-canonical properties. It is located in the sperm head rather than the flagellum and is controlled by intracellular pH, but not cyclic nucleotides. Alkalization hyperpolarizes sperm and produces Ca2+ entry. Ca2+ induces spinning-like swimming, different from swimming of sperm from other species. The “spinning” mode probably guides sperm into the micropyle, a narrow entrance on the surface of fish eggs. A picture is emerging of sperm channel orthologues that employ different activation mechanisms and serve different functions. The channel inventories probably reflect adaptations to species-specific challenges during fertilization. |