This title appears in the Scientific Report :
2015
Please use the identifier:
http://dx.doi.org/10.1021/acs.analchem.5b00617 in citations.
Monitoring of the Enzymatically Catalyzed Degradation of Biodegradable Polymers by Means of Capacitive Field-Effect Sensors
Monitoring of the Enzymatically Catalyzed Degradation of Biodegradable Polymers by Means of Capacitive Field-Effect Sensors
Designing novel or optimizing existing biodegradable polymers for biomedical applications requires numerous tests on the effect of substances on the degradation process. In the present work, polymer-modified electrolyte–insulator–semiconductor (PMEIS) sensors have been applied for monitoring an enzy...
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Personal Name(s): | Schusser, Sebastian |
---|---|
Krischer, Maximilian / Bäcker, Matthias / Poghossian, Arshak (Corresponding author) / Wagner, Patrick / Schöning, Michael J. | |
Contributing Institute: |
Bioelektronik; PGI-8 |
Published in: | Analytical chemistry, 87 (2015) 13, S. 6607 - 6613 |
Imprint: |
Columbus, Ohio
American Chemical Society
2015
|
PubMed ID: |
26016927 |
DOI: |
10.1021/acs.analchem.5b00617 |
Document Type: |
Journal Article |
Research Program: |
Controlling Configuration-Based Phenomena |
Publikationsportal JuSER |
Designing novel or optimizing existing biodegradable polymers for biomedical applications requires numerous tests on the effect of substances on the degradation process. In the present work, polymer-modified electrolyte–insulator–semiconductor (PMEIS) sensors have been applied for monitoring an enzymatically catalyzed degradation of polymers for the first time. The thin films of biodegradable polymer poly(d,l-lactic acid) and enzyme lipase were used as a model system. During degradation, the sensors were read-out by means of impedance spectroscopy. In order to interpret the data obtained from impedance measurements, an electrical equivalent circuit model was developed. In addition, morphological investigations of the polymer surface have been performed by means of in situ atomic force microscopy. The sensor signal change, which reflects the progress of degradation, indicates an accelerated degradation in the presence of the enzyme compared to hydrolysis in neutral pH buffer media. The degradation rate increases with increasing enzyme concentration. The obtained results demonstrate the potential of PMEIS sensors as a very promising tool for in situ and real-time monitoring of degradation of polymers. |