This title appears in the Scientific Report :
2015
Please use the identifier:
http://dx.doi.org/10.1002/pssa.201431900 in citations.
Towards a biosensor to monitor the sterilisation efficiency of aseptic filling machines
Towards a biosensor to monitor the sterilisation efficiency of aseptic filling machines
Sterilisation processes are compulsory in medicine, pharmacy, and food industries to prevent infections of consumers and microbiological contaminations of products. Monitoring the sterilisation by conventional microbiological methods is time- and lab-consuming. To overcome this problem, in this work...
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Personal Name(s): | Oberländer, Jan |
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Bromm, Alexander / Wendeler, Luisa / Iken, Heiko / Durán, Marlena Palomar / Greeff, Anton / Kirchner, Patrick / Keusgen, Michael / Schöning, Michael J. (Corresponding author) | |
Contributing Institute: |
Bioelektronik; PGI-8 |
Published in: | Physica status solidi / A, 212 (2015) 6, S. 1299 - 1305 |
Imprint: |
Weinheim
Wiley-VCH
2015
|
DOI: |
10.1002/pssa.201431900 |
Document Type: |
Journal Article |
Research Program: |
Controlling Configuration-Based Phenomena |
Publikationsportal JuSER |
Sterilisation processes are compulsory in medicine, pharmacy, and food industries to prevent infections of consumers and microbiological contaminations of products. Monitoring the sterilisation by conventional microbiological methods is time- and lab-consuming. To overcome this problem, in this work a novel biosensor has been proposed. The sensor enables a fast method to evaluate sterilisation processes. By means of thin-film technology the sensor's transducer structures in form of IDEs (interdigitated electrodes) have been fabricated on a silicon substrate. Physical characterisation of the developed sensor was done by AFM, SEM, and profilometry. Impedance analyses were conducted for the electrical characterisation. As microbiological layer spores of B. atrophaeus have been immobilised on the sensing structure; spores of this type are a well-known sterilisation test organism. Impedance measurements at a fixed frequency over time were performed to monitor the immobilisation process. A sterilisation process according to aseptic filling machines was applied to demonstrate the sensor functionality. After both, immobilisation and sterilisation, a change in impedance could successfully be detected. |