This title appears in the Scientific Report :
2015
Please use the identifier:
http://dx.doi.org/10.1002/cctc.201500645 in citations.
Combination of Glycosyltransferases and a Glycosynthase in Sequential and One-Pot Reactions for the Synthesis of Type 1 and Type 2 N -Acetyllactosamine Oligomers
Combination of Glycosyltransferases and a Glycosynthase in Sequential and One-Pot Reactions for the Synthesis of Type 1 and Type 2 N -Acetyllactosamine Oligomers
The disaccharide units of N-acetyllactosamine (LacNAc) types 1 and 2 and poly-LacNAc are involved in many biological recognition events. We report the modular design of type 1 (3Galβ1-3GlcNAcβ1-; Gal=d-galactose, GlcNAc=N-acetyl d-glucosamine) and type 2 (3Galβ1-4GlcNAcβ1-) LacNAc oligomers by combi...
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Personal Name(s): | Henze, Manja |
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Schmidtke, Simon / Hoffmann, Natalie / Steffens, Hanna / Pietruszka, Jörg / Elling, Lothar (Corresponding author) | |
Contributing Institute: |
Institut für Bioorganische Chemie (HHUD); IBOC Biotechnologie; IBG-1 |
Published in: | ChemCatChem, 7 (2015) 19, S. 3131 - 3139 |
Imprint: |
Weinheim
WILEY-VCH Verlag
2015
|
DOI: |
10.1002/cctc.201500645 |
Document Type: |
Journal Article |
Research Program: |
Biotechnology |
Publikationsportal JuSER |
The disaccharide units of N-acetyllactosamine (LacNAc) types 1 and 2 and poly-LacNAc are involved in many biological recognition events. We report the modular design of type 1 (3Galβ1-3GlcNAcβ1-; Gal=d-galactose, GlcNAc=N-acetyl d-glucosamine) and type 2 (3Galβ1-4GlcNAcβ1-) LacNAc oligomers by combination of two recombinant glycosyltransferases, β1,3-N-acetylglucosaminyltransferase from Helicobacter pylori and human β1,4-galactosyltransferase-1, with galactosynthase His6BgaC/Glu233Gly from Bacillus circulans. Combination of His6BgaC/Glu233Gly and β1,3-N-acetylglucosaminyltransferase in sequential or one-pot mode results in LacNAc type 1 oligomers with up to four LacNAc units. Moreover, sequential use of all three enzymes gives access to a variety of neo-LacNAc oligomers. We present oligomers composed of alternating type 1 and 2 units and type 1-terminated LacNAc type 2 oligomers. These glycan structures are valuable compounds for testing carbohydrate–protein interactions. |