This title appears in the Scientific Report :
2016
Please use the identifier:
http://dx.doi.org/10.1002/elsc.201500056 in citations.
Simplified cryopreservation of the microalga Chlorella vulgaris integrating a novel concept for cell viability estimation
Simplified cryopreservation of the microalga Chlorella vulgaris integrating a novel concept for cell viability estimation
Microalgae currently receive growing attention as promising candidates for future bio-economy concepts. However, the reliable maintenance of production strains remains challenging. The well-established serial subculturing techniques suffer from low long-time stability and high effort and are therefo...
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Personal Name(s): | Morschett, Holger |
---|---|
Reich, Sebastian / Wiechert, Wolfgang / Oldiges, Marco (Corresponding author) | |
Contributing Institute: |
Biotechnologie; IBG-1 |
Published in: | Engineering in life sciences, 16 (2016) 1, S. 36-44 |
Imprint: |
Weinheim
Wiley-VCH
2016
|
DOI: |
10.1002/elsc.201500056 |
Document Type: |
Journal Article |
Research Program: |
Innovative Synergisms |
Publikationsportal JuSER |
Microalgae currently receive growing attention as promising candidates for future bio-economy concepts. However, the reliable maintenance of production strains remains challenging. The well-established serial subculturing techniques suffer from low long-time stability and high effort and are therefore stepwise being replaced by cryopreservation. Currently, available protocols are often deduced from cell culture technology and are rather complex. This study aimed to investigate if less complex approaches can be applied. We introduce an easy-to-use cryopreservation protocol based on the model organism Chlorella vulgaris. To overcome error-prone viability estimation by plating techniques, an alternative method using growth pattern analysis was developed. As revealed by growth pattern analysis, the preservation of stationary phase cells proved superior to the commonly applied concept of freezing cells from the growing phase. Controlled-rate cooling using simple devices resulted in reproducibly high post-thawing viabilities in the range of 63 ± 2%. Moreover, the presented protocol highlights the potential of simplifying microalgal cryo-preservation procedures, thereby reducing the required labor and material need to a minimum. Apart from the viability analysis of the cryopreserved microalga C. vulgaris, this approach seems to have the potential to be applied for other algae species and microorganisms, as well. |