This title appears in the Scientific Report :
2003
Please use the identifier:
http://dx.doi.org/10.1021/bp034016f in citations.
Please use the identifier: http://hdl.handle.net/2128/2072 in citations.
Micropatterned substrates for the growth of functional neuronal networks of defined geometry
Micropatterned substrates for the growth of functional neuronal networks of defined geometry
The in vitro assembly of neuronal networks with control over cell position and connectivity is a fascinating approach not only for topics in basic neuroscience research but also in diverse applications such as biosensors and tissue engineering. We grew rat embryonic cortical neurons on patterned sub...
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Personal Name(s): | Vogt, A. |
---|---|
Lauer, L. / Knoll, W. / Offenhäusser, A. | |
Contributing Institute: |
Institut für Bio- und Chemosensoren; ISG-2 |
Published in: | Biotechnology progress, 19 (2003) S. 1562 - 1568 |
Imprint: |
Malden, MA
Wiley
2003
|
Physical Description: |
1562 - 1568 |
DOI: |
10.1021/bp034016f |
PubMed ID: |
14524720 |
Document Type: |
Journal Article |
Research Program: |
Materialien, Prozesse und Bauelemente für die Mikro- und Nanoelektronik |
Series Title: |
Biotechnology Progress
19 |
Subject (ZB): | |
Link: |
Get full text OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/2072 in citations.
The in vitro assembly of neuronal networks with control over cell position and connectivity is a fascinating approach not only for topics in basic neuroscience research but also in diverse applications such as biosensors and tissue engineering. We grew rat embryonic cortical neurons on patterned substrates created by microcontact printing. Polystyrene was used as a cell repellent background, onto which a grid pattern of physiological proteins was applied. We printed laminin and a mixture of extracellular matrix proteins and additionally both systems mixed with polylysine. Attachment of cells to the pattern with high fidelity as well as the formation of chemical synapses between neighboring cells on the pattern could be observed in all four cases, but cell attachment was strongly increased on samples containing polylysine. Neurons grown on patterned substrates had a membrane capacity smaller than that of neurons on homogeneously coated controls, which we attributed to the geometrical restrictions, but did not differ either in resting membrane potential or in the quality of synapses they formed. We therefore believe that the cells attach and differentiate normally on the pattern and form functional, mature synapses following the predefined geometry. |