This title appears in the Scientific Report :
2004
Please use the identifier:
http://dx.doi.org/10.1007/s00253-003-1455-8 in citations.
Biotransformation of glucose to 5-keto-D-gluconic acid by recombinant Gluconobacter oxydans DSM 2343
Biotransformation of glucose to 5-keto-D-gluconic acid by recombinant Gluconobacter oxydans DSM 2343
For the conversion of glucose to 5-keto-D-gluconate (5-KGA), a precursor of the industrially important L-(+)-tartaric acid, Gluconobacter strains were genetically engineered. In order to increase 5-KGA formation, a plasmid-encoded copy of the gene encoding the gluconate:NADP-5 oxidoreductase (gno) w...
Saved in:
Personal Name(s): | Herrmann, U. |
---|---|
Merfort, M. / Jeude, M. / Bringer-Meyer, S. / Sahm, H. | |
Contributing Institute: |
Biotechnologie 1; IBT-1 |
Published in: | Applied Microbiology and Biotechnology, 64 (2004) S. 86 - 90 |
Imprint: |
Berlin
Springer
2004
|
Physical Description: |
86 - 90 |
PubMed ID: |
14564486 |
DOI: |
10.1007/s00253-003-1455-8 |
Document Type: |
Journal Article |
Research Program: |
Biotechnologie |
Series Title: |
Applied Microbiology and Biotechnology
64 |
Subject (ZB): | |
Publikationsportal JuSER |
For the conversion of glucose to 5-keto-D-gluconate (5-KGA), a precursor of the industrially important L-(+)-tartaric acid, Gluconobacter strains were genetically engineered. In order to increase 5-KGA formation, a plasmid-encoded copy of the gene encoding the gluconate:NADP-5 oxidoreductase (gno) was overexpressed in G. oxydans strain DSM 2434. This enzyme is involved in the nonphosphorylative ketogenic oxidation of glucose and oxidizes gluconate to 5-KGA. As the 5-KGA reductase activity depends on the cofactor NADP+, the sthA gene (encoding Escherichia coli transhydrogenase) was cloned and overexpressed in the GNO-overproducing G. oxydans strain. Growth of the sthA-carrying strains was indistinguishable from the G. oxydans wild-type strain and therefore they were chosen for the coupled overexpression of sthA and gno. G. oxydans strain DSM 2343/pRS201-gno-sthA overproducing both enzymes showed an enhanced accumulation of 5-KGA. |